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Anti cd45 hi30

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Anti-CD45 (HI30) is a monoclonal antibody that binds to the CD45 antigen, which is expressed on the surface of most human leukocytes. The antibody can be used for the identification and enumeration of leukocyte populations in flow cytometry applications.

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Lab products found in correlation

Facscanto 2 flow cytometer, by BD (1 mentions) Bodipy 493 503, by Thermo Fisher Scientific (1 mentions) Collagenase 2, by Thermo Fisher Scientific (1 mentions) Lipidtox deep red, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Fixation and permeabilization solution kit, by BD (1 mentions) Anti cd127 a019d5, by BioLegend (1 mentions) Anti arginase 1, by R&D Systems (1 mentions) Human fcr binding inhibitor, by Thermo Fisher Scientific (1 mentions) Anti cd40 5c3, by BioLegend (1 mentions) Anti mmp 9, by R&D Systems (1 mentions)

Spelling variants of this product

Anti-CD45 (119 citations) CD45 (HI30) (6 citations) Anti-human CD45 (clone HI30; (2 citations)

3 protocols using anti cd45 hi30

1

Isolation and Characterization of Human Visceral Adipose Tissue

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Human male and female visceral adipose tissue was collected during bariatric surgery of patients with obesity (See Figure 7C) with Institutional Review Board approval (HUM00074075) from the University of Michigan and Ann Arbor Veterans Administration Hospital. Tissue was finely minced by hand using surgical scissors (DR Instruments, 4SB), then digested in 1 mg/ml collagenase II (Life Technologies, 17101015) for 1 hour and processed as for mouse adipose tissue to obtain single cell suspensions of SVCs. Cells were then stained with anti-CD45 (HI30) from eBioscience, and anti-CD64 (10.1) and anti-CD11c (3.9) from Biolegend. Neutral lipids were detected using LipidTOX Deep Red as for mouse cells, and Bodipy 493/503 (Life Technologies, D3922) for imaging flow cytometry. Flow cytometry data were collected using a BD FACSCanto II flow cytometer and data were analyzed as for mouse cells using similar gating (Supplemental Figure S1A). Imaging flow cytometry data were collected on an ImagestreamX Mark II and analyzed using IDEAS software v6.0 (Amnis).
Muir L.A., Kiridena S., Griffin C., DelProposto J.B., Geletka L., Martinez-Santibañez G., Zamarron B.F., Lucas H., Singer K., O’Rourke R.W, & Lumeng C.N. (2018). Rapid adipose tissue expansion triggers unique proliferation and lipid accumulation profiles in adipose tissue macrophages. Journal of leukocyte biology, 103(4), 615-628.
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2

Characterizing Immune Cell Phenotypes

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Human FcR Binding Inhibitor (eBioscience) was added to the samples (1:200) and incubated for 20 min on ice to block non-specific Fc-receptor binding. After, cells were washed with FACS buffer and stained with surface antibodies, which included anti-CD45 (HI30, eBioscience); anti-CD40 (5C3, BioLegend); and anti-CD127 (A019D5, BioLegend), at room temperature for 15 min in the dark. Samples were washed twice with FACS buffer. Intracellular staining with anti-CD68 (eBioY1/82A, eBioscience), anti-arginase-1 (R&D systems), and anti-MMP9 (R&D systems) was performed using the Fixation and Permeabilization Solution kit (BD Biosciences). Dead cells were excluded using 7-AAD staining. Samples were run on BD LSRII flow cytometer and data were analyzed using FACSDiva software and FlowJo.
Jiang X., Wang M., Cyrus N., Yanez D.A., Lacher R.K., Rhebergen A.M., Brokowski C., Galan A., Book S, & Colegio O.R. (2019). Human keratinocyte carcinomas have distinct differences in their tumor-associated macrophages. Heliyon, 5(8), e02273.
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3

Immunohistochemical Analysis of Skin Biopsies

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CLE skin biopsies from lesions of patients with ACLE, SCLE, or DLE as well as healthy controls were collected and fixed in formalin. Formalin-fixed, paraffin-embedded skin biopsy sections were assayed by chromogenic immunostaining for pan-leukocytes (anti-CD45, HI30, eBioscience), B cells (anti-CD20, L26, Abcam) and memory B cells (anti-CD27, polyclonal, R&D Systems). Antigen retrieval was achieved by heating sections in sodium citrate buffer (pH 6.0) prior to antibody incubation. A minimum of 3 patients per disease status group were assayed and representative images are shown.
Abernathy-Close L., Lazar S., Stannard J., Tsoi L.C., Eddy S., Rizvi S.M., Yee C.M., Myers E.M., Namas R., Lowe L., Reed T.J., Wen F., Gudjonsson J.E., Kahlenberg J.M, & Berthier C.C. (2021). B Cell Signatures Distinguish Cutaneous Lupus Erythematosus Subtypes and the Presence of Systemic Disease Activity. Frontiers in Immunology, 12, 775353.
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