Q tof ultima global gaa076lc

Optical rotations were measured on a JASCO P-1020 digital polarimeter. UV spectra were recorded on a Beckman DU640 spectrophotometer. ECD spectra were obtained on an Applied Photophysics Chirascan spectropolarimeter. IR spectra were taken on a Nicolet NEXUS 470 spectrophotometer in KBr disks. NMR spectra were measured on a Bruker AVANCE 500 spectrometer. The 7.26 ppm and 77.2 ppm resonances of CDCl₃ were used as internal references for ¹H and ¹³C NMR spectra, respectively. HRESIMS data were measured on Micromass Q-Tof Ultima Global GAA076LC and Thermo Scientific LTQ orbitrap XL mass spectrometers. Semi-preparative HPLC utilized an ODS column (YYMC-Pack ODS-A, 10 × 250 mm, 5 µm, 1.5 mL/min). Silica gel (200-300 mesh, Qingdao) was used for column chromatography, and precoated silica gel plates (GF254, Qingdao) were used for TLC, and spots visualized by heating SiO₂ plates sprayed with 5% H₂SO₄ in EtOH.

Optical rotations were measured on a Jasco P-1020 digital polarimeter. UV spectra were recorded on a Beckman DU640 spectrophotometer. CD spectra were obtained on a Jasco J-810 spectropolarimeter. IR spectra were taken on a Nicolet NEXUS 470 spectrophotometer in KBr discs. NMR spectra were measured by JEOL JNMECP 600 and Bruker AVANCE III 600 spectrometers. The 7.2600 and 3.3100 ppm resonances of residual CDCl₃ and CD₃OD, and 77.16 and 49.00 ppm resonances of CDCl₃ and CD₃OD were used as internal references for ¹H and ¹³C NMR spectra, respectively. HRESIMS spectra were measured on a Micromass Q-Tof Ultima GLOBAL GAA076 LC and Thermo Scientific LTQ orbitrap XL mass spectrometers. Semi-preparative HPLC utilized an ODS column [YMC-Pack ODS-A, 10 × 250 mm, 5 μm, 1.5 mL/min]. Chiral HPLC utilized chiral analytical columns [Daicel Chiralpack IB and IC: 5 μm, 4.6 mm × 250 mm]. Silica gel (200–300 mesh, Qingdao, China) was used for column chromatography, and pre-coated Silica gel plates (GF254, Qingdao, China) were used for TLC, and spots visualized by heating SiO₂ plates sprayed with 5% H₂SO₄ in EtOH.

Optical rotations were measured on a JASCO P-1020 digital polarimeter. UV spectra were recorded on a Beckman DU640 spectrophotometer. CD spectra were obtained on a JASCO J-810 spectropolarimeter. IR spectra were taken on a Nicolet NEXUS 470 spectrophotometer in KBr discs. NMR spectra were measured by Bruker AVANCE III 600 spectrometers. The 2.5000 ppm and 39.50 ppm resonances of DMSO were used as internal references for ¹H and ¹³C NMR spectra, respectively. HRESIMS spectra were measured on a Micromass Q-Tof Ultima GLOBAL GAA076LC and Thermo Scientific LTQ orbitrap XL mass spectrometers. Semi-preparative HPLC utilized an ODS column [YYMC-Pack ODS-A, 100 × 250 mm, 5 µm, 1.5 mL/min]. Chiral HPLC utilized chiral analytical columns [CHIRALPAK IC column (4.6 × 250 mm, 5 µm)]. Silica gel (200–300 mesh, Qingdao, China) was used for column chromatography, and precoated Silica gel plates (GF254, Qingdao, China) were used for TLC, and spots visualized by heating SiO₂ plates sprayed with 5% H₂SO₄ in EtOH.