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Ab205926

Manufactured by Abcam
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Ab205926 is a recombinant antibody product manufactured by Abcam. It is a laboratory tool intended for research use.

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Lab products found in correlation

Rabbit primary antibody, by Cell Signaling Technology (2 mentions)

2 protocols using ab205926

1

Detailed Western Blot Protocol for p38 MAPK

Cited 1 time
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Western blots were performed as described previously (Bliss-Moreau et al., 2015 (link)). Rabbit primary antibodies (Cell Signaling Technology [CST], Danvers, MA) were used at the following dilutions: anti-p38α, -p38β, -p38γ, and -p38δ (1:1,000); anti-β-tubulin (1:2,000), anti-p38 GAPDH 1:1,000; anti-β-actin (8H10D10) mouse mAb (1:2,000, CST); anti-DLGH1 total protein; and anti-pDLGH1 at serine 158 and 431 (total SAP97, S285B SAP97 phospho-Ser158 and 431, affinity purified sheep polyclonal antibody, University of Dundee, Scotland, 1:1;000). horseradish peroxidase-conjugated goat anti-rabbit antibody #7074 and anti-mouse IgG horseradish peroxidase-linked antibody #7076 (CST, 1:2,000) were used as secondary antibodies. For detection of p38γ protein level in SS patient samples, we used a monoclonal antibody that close to N-terminus of human p38γ MAPK (SAPK3) from Abcam (ab205926).
Zhang X.H., Nam S., Wu J., Chen C.H., Liu X., Li H., McKeithan T., Gong Q., Chan W.C., Yin H.H., Yuan Y.C., Pillai R., Querfeld C., Horne D., Chen Y, & Rosen S.T. (2018). Multi-Kinase Inhibitor with Anti-p38γ Activity in Cutaneous T-Cell Lymphoma. The Journal of investigative dermatology, 138(11), 2377-2387.
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2

Detailed Western Blot Protocol for p38 MAPK

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots were performed as described previously (Bliss-Moreau et al., 2015 (link)). Rabbit primary antibodies (Cell Signaling Technology [CST], Danvers, MA) were used at the following dilutions: anti-p38α, -p38β, -p38γ, and -p38δ (1:1,000); anti-β-tubulin (1:2,000), anti-p38 GAPDH 1:1,000; anti-β-actin (8H10D10) mouse mAb (1:2,000, CST); anti-DLGH1 total protein; and anti-pDLGH1 at serine 158 and 431 (total SAP97, S285B SAP97 phospho-Ser158 and 431, affinity purified sheep polyclonal antibody, University of Dundee, Scotland, 1:1;000). horseradish peroxidase-conjugated goat anti-rabbit antibody #7074 and anti-mouse IgG horseradish peroxidase-linked antibody #7076 (CST, 1:2,000) were used as secondary antibodies. For detection of p38γ protein level in SS patient samples, we used a monoclonal antibody that close to N-terminus of human p38γ MAPK (SAPK3) from Abcam (ab205926).
Zhang X.H., Nam S., Wu J., Chen C.H., Liu X., Li H., McKeithan T., Gong Q., Chan W.C., Yin H.H., Yuan Y.C., Pillai R., Querfeld C., Horne D., Chen Y, & Rosen S.T. (2018). Multi-Kinase Inhibitor with Anti-p38γ Activity in Cutaneous T-Cell Lymphoma. The Journal of investigative dermatology, 138(11), 2377-2387.
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