OGAinh thiamet G was obtained from Tocris Bioscience (Bristol, UK), while OGT inhibitors (OGTinh) alloxan and OSMI-1 were from Sigma-Aldrich (St. Louis, MO). Dimethyl sulfoxide (DMSO; Merck, Darmstadt, Germany) was used as a solvent for thiamet G and OSMI-1 to prepare 1000X stock solutions, and 0.1% DMSO was used as a vehicle (nontreated) control. Antibodies for globins were obtained from Santa Cruz Biotechnology (Dallas, Texas), while other antibodies were from Abcam (Cambridge, UK), unless otherwise mentioned. All cytokines were obtained from Miltenyi (Bergisch Gladbach, Germany). Full details of key resources can be found in Additional file 1 : Table S1.
Other antibodies
Manufactured by Abcam
Sourced in United States
Other antibodies are a diverse range of immunoglobulin-based reagents used in various applications, such as research, diagnostics, and therapeutic development. These antibodies target a wide array of biomolecules and can be used for techniques like immunoassays, immunoprecipitation, and flow cytometry. The core function of these antibodies is to provide specific binding and detection of target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Osmi 1, by Merck Group (1 mentions)
Chemicapture software, by Clinx (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Acrylamide bisacrylamide, by Bio-Rad (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Immunoblotting membrane, by Bio-Rad (1 mentions)
ε amino n caproic acid, by MP Biomedicals (1 mentions)
Bradford protein assay solution, by Bio-Rad (1 mentions)
Nbt chloride tablets, by Merck Group (1 mentions)
Serva blue g, by Serva Electrophoresis (1 mentions)
Rabbit anti dldh polyclonal antibodies igg, by US Biological (1 mentions)
Goat anti rabbit igg conjugated with horseradish peroxidase, by US Biological (1 mentions)
Ammonium persulfate, by Bio-Rad (1 mentions)
Coomassie brilliant blue r 250, by Bio-Rad (1 mentions)
Lipoamide, by Merck Group (1 mentions)
Hybond ecl nitrocellulose membrane, by Cytiva (1 mentions)
Hybond, by Cytiva (1 mentions)
4 protocols using other antibodies
1
Comprehensive Protocol for O-GlcNAc Inhibition
2
Western Blot Analysis of Protein Expression
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An equal amount of protein (30 mg) from cell lysate was loaded into each well of a 12% SDS-polyacrylamide gel after denaturation with SDS loading buffer. After electrophoresis, proteins were transferred to a PVDF membrane, incubated with blocking buffer (5% fat-free milk) for 1 h at room temperature, and blotted with the following antibodies overnight: Anti-GAPDH (Cell Signaling Technology) and other antibodies (Abcam). The membrane was incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. All signals were visualized and analyzed by Clinx ChemiCapture software (Clinx, Shanghai, China)
3
Protein Carbonyl Assay Protocol
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Biotin-linked aldehyde reactive probe ARP) for protein carbonyl assay was from Cayman Chemical (Ann Arbor, MI). Dihydrolipoamide was synthesized from lipoamide in our own laboratory using sodium borohydride as previously described [29] , [30] . ε-amino-N-caproic acid was obtained from MP Biochemicals. Acrylamide/bisacrylamide, ammonium persulfate, Bradford protein assay solution, coomassie brilliant blue (CBB) R-250, immunoblotting membrane, and an ECL immunochemical detection kit were from Bio-Rad laboratories (Richmond, CA, USA). NADH, BSA, lipoamide, EDTA, ATP, and NBT chloride tablets were obtained from Sigma (St. Louis, MO, USA). Serva Blue G was purchased from Serva (Heidelberg, Germany). Anti-PARP antibody was purchased from Trevigen (Gaithers burg, MD). Anti-NQO1 antibodies were from Sigma. Rabbit anti-DLDH polyclonal antibodies (IgG) and goat anti-rabbit IgG conjugated with horseradish peroxidase were purchased from US Biological (Swampscott, MA, USA) and Invitrogen (San Diego, CA, USA), respectively. Other antibodies were from Abcam (Cambridge, MA).
4
Integrin β4 Expression Analysis
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Equal amounts of protein extracts were separated by SDS-PAGE, and transferred onto Hybond ECL nitrocellulose membranes (Amersham Life Science, Buckinghamshire, UK). After non-speci c binding was blocked using 5% bovine serum albumin in TBST for 1 h, membranes were incubated overnight with antiintegrin β4 antibody (Cell Signalling Technology Inc. Danvers, MA, USA), or other antibodies (Abcam, Waltham, MA, USA), at 4°C. Then, the membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibody. β-Actin was used as loading control. Chemiluminescence was detected using ECL reagent on a luminescent image analyser, LAS-4000 mini.
For co-IP, total proteins were lysed in IP lysis buffer (Thermo Fisher Scienti c; Waltham, MA, USA).
For co-IP, total proteins were lysed in IP lysis buffer (Thermo Fisher Scienti c; Waltham, MA, USA).
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