Trans nonachlor

Manufactured by Merck Group

Trans-nonachlor is a laboratory chemical compound used as a standard in analytical procedures. It is a chlorinated organic compound that can be used as a reference material for the identification and quantification of similar substances in various samples.

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Lab products found in correlation

Cell counting kit 8 (cck8), by Merck Group (1 mentions) Glutathione (gsh), by Cayman Chemical (1 mentions) 4 4 difluoro 5 methyl 4 bora 3a 4a diaza s indacene 3 dodecanoic acid bodipy 500 510 c12 bodipy fa, by Thermo Fisher Scientific (1 mentions) β hydroxybutyrate β hb, by Cayman Chemical (1 mentions) Tbars, by Cayman Chemical (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Rifampicin, by Merck Group (1 mentions) Sr12813, by Merck Group (1 mentions) Spa70, by WuXi AppTec (1 mentions) T0901317, by Merck Group (1 mentions) 17α ethinylestradiol ee2, by Merck Group (1 mentions)

2 protocols using trans nonachlor

Assessing Hepatocyte Lipid Metabolism

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McArdle-RH7777 (McA) rat hepatoma cells were obtained from American Type
Culture Collection (ATCC) and grown in DMEM with high glucose, 20% FBS,
sodium pyruvate, glutamine, penicillin, and streptomycin (normal growth media)
for routine culturing. Trans-nonachlor (>99% purity), oleic acid,
sodium palmitate, Oil Red O stain, Janus Green stain, and cell viability reagent
(Cell Counting Kit-8) were obtained from Sigma Aldrich. Trans-nonachlor stock
solutions were made in dimethylsulfoxide (DMSO) at a 4000x concentration to
produce DMSO (0.025%) as the final DMSO concentration in vehicle
controls. 4,4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid
(BODIPY 500/510 C12; BODIPY-FA) was obtained from Life Technologies for use in
fatty acid uptake assays. ¹⁴C-acetate was purchased from Perkin Elmer
for use in DNL assays. TBARS, glutathione, β-hydroxybutyrate
(β-HB), and triglyceride assay kits were purchased from Cayman
Chemical.

Howell GE I.I.I., McDevitt E., Henein L., Mulligan C, & Young D. (2018). Trans-nonachlor increases extracellular free fatty acid accumulation and de novo lipogenesis to produce hepatic steatosis in McArdle-RH7777 cells. Toxicology in vitro : an international journal published in association with BIBRA, 50, 285-292.

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Chemical Compound Acquisition for Experimental Research

Cited 1 time

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DMSO, rifampicin, T0901317, SR12813, 17α-ethinylestradiol (EE2), and trans-nonachlor were purchased from Sigma-Aldrich. SPA70, SJB7, and SJC2 were obtained from WuXi AppTec [16 (link)]. All oligonucleotides were from Integrated DNA Technologies and are presented in Supplementary Table 2.

Huber A.D., Wright W.C., Lin W., Majumder K., Low J.A., Wu J., Buchman C.D., Pintel D.J, & Chen T. (2020). Mutation of a single amino acid of pregnane X receptor switches an antagonist to agonist by altering AF-2 helix positioning. Cellular and molecular life sciences : CMLS, 78(1), 317-335.

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