Sodium arsenate na2haso4

The S. cerevisiae strains used in this study are based on BY4742 (MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0) (Brachmann et al. 1998 (link)) and the following mutants from yeast deletion collection (Winzeler et al. 1999 (link)): etp1∆ (BY4742 etp1∆::kanMX) and yap8∆ (BY4742 yap8∆::kanMX). The etp1∆ yap8∆ double mutant (BY4742 etp1∆::kanMX yap8∆::kanMX) was generated by crossing haploid single mutants using standard procedures. Yeast cells were routinely grown at 30°C on minimal SC (synthetic complete) medium (0.67% yeast nitrogen base; YNB) supplemented with auxotrophic requirements and 2% glucose as a carbon source. Sodium arsenite (NaAsO₂) (Sigma-Aldrich, St. Louis, MO, USA), sodium arsenate (Na₂HAsO₄) (Sigma-Aldrich ) and cycloheximide (Sigma-Aldrich) were added directly to the growth medium. Plate growth assays were performed as previously described (Wysocki et al. 2001 (link)).
The plasmids used in this study include Yap8-HA under the control of the constitutive TPI1 promoter in pYX122 (CEN, HIS3) (Di and Tamás 2007 (link)), GFP-Yap8 controlled by the endogenous YAP8 promoter in YEplac195 (2μ, URA3) (Wysocki et al. 2004 (link)), p415TEF1-10×His-Ub-LEU2 (pGR295; kindly provided by Gwenaël Rabut), pES15 ACR3-lacZ (CEN, URA3) (Wysocki et al. 2004 (link)) and pA103 ACR3 (2µ, URA3) (Bobrowicz et al. 1997 (link)).