Purelab option q system

Aluminum foils of 99.999% of purity and 0.5 mm of thickness were purchased from Goodfellow Ltd. (Cambridge, UK). Oxalic acid (H₂C₂O₄), phosphoric acid (H₃PO₄), perchloric acid (HClO₄), chromic acid (H₂CrO₇), copper chloride (CuCl₂) ethanol (C₂H₅OH), acetone ((CH₃)₂CO), 2-(N-morpholino)ethanesulfonic acid, phosphate buffered saline (PBS), human serum albumin (HSA), streptavidin, (3-aminopropyl)triethoxysilane (APTES), sulfo-NHS-biotin, magnesium chloride (MgCl₂) and thrombin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Biotin modified aptamer 15-mer (5′biotin-GGT TGG TGT GGT TGG-3′) was purchased from Eurofins Genomic GmbH (Ebersberg, Germany). Double-deionized (DI) water Double-deionized (DI) water in 18.6 MU, PURELAB Option-Q system purchased from ELGA LabWater (Lane End, United Kindom) was used for all solutions.

The reference standard (RS) of KAU (purity > 98.6%) and honokiol used as an internal standard (IS) (Figure 1) were obtained from the Ministry of Food and Drug Safety (Cheongju-si, Chungcheongbuk-do, Korea). Methanol, acetonitrile, and methyl-tert-butyl ether were purchased from J.T. Baker (Phillipsburg, NJ, USA). Ammonium acetate, formic acid, and trifluoroacetic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade water (18.2 MΩ) was obtained using an Elga Purelab option-Q system (Elga Labwater, Marlow, UK). It was used throughout this study. Other chemicals were of HPLC grade or the highest quality available.

All chemicals used for the extraction and analysis of FAs, VitE, and phenolic compounds were of the HPLC-grade or at least ACS analytical grade. Acetonitrile, ethanol, methanol (MeOH), and iso-octane were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Deionized water was generated using the PURELAB Option-Q System (ELGA Lab Water, High Wycombe, UK). Potassium hydroxide, benzene, formic acid, and heptane were purchased from Junsei (Tokyo, Japan). Hexane was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Dichloromethane, 2,2-dimethoxypropane, and dimethyl sulfoxide were purchased from Sigma-Aldrich Corp (St. Louis, MO, USA). Sulfuric acid, sodium sulfate anhydrous, and 0.1 N hydrochloric acid were purchased from Daejung Chemical and Materials Co., Ltd. (Gyeonggi-Do, Korea). Ascorbic acid was obtained from SANCHUN Chemical Co., Ltd. (Gyeonggi-Do, Korea). The 37 fatty acid methyl ester (FAME) standard mixture, pentadecanoic acid (C15:0, internal standard), VitE (4 tocopherols and 4 tocotrienols), and phenolic standards (STDs) were phurchased from Sigma-Aldrich Co. We obtained 2,2-diphenyl-1-picrylhydrazyl (DPPH) from Alfa Aesar (Ward Hill, MA, USA).