Apc anti human cd117

Manufactured by BioLegend

Sourced in United States

APC anti-human CD117 is a monoclonal antibody that binds to the CD117 antigen, also known as c-Kit, on the surface of human cells. CD117 is a tyrosine kinase receptor that plays a role in the regulation of cell growth, differentiation, and survival. This product is intended for research use only.

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Lab products found in correlation

Ma900 sorter, by Sony (2 mentions) Ficoll, by BD (2 mentions) Pe anti human cd45, by BioLegend (2 mentions) Lsrfortessa, by BD (1 mentions) Aldefluor kit, by STEMCELL (1 mentions) Lsrfortessa x 20, by BD (1 mentions)

4 protocols using apc anti human cd117

ALDH Activity and Ovarian CSC Phenotype

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ALDH enzymatic activity was assessed using the ALDEFLUOR kit (Stem Cell Technologies, Vancouver, BC, Canada) following the manufacturer’s protocol. Cells treated with ALDH inhibitor DEAB were used as a negative control. The surface markers of ovarian cancer stem cells CD44 (FITC anti-human CD44; Cat. No. 338804; Biolegend, San Diego, CA, USA) and CD117 (APC anti-human CD117; Cat. No. 313206; Biolegend) were also stained for determination of cancer stem cells. Flow cytometry (BD LSRFortessa™) was applied to analyze the ALDH⁺ and CD44⁺CD117⁺ cells.

Ruan S., Zhang H., Tian X., Zhang Z., Huang H., Shi C., Liu W., Jiang X., Huang D, & Tao F. (2020). PHD Finger Protein 19 Enhances the Resistance of Ovarian Cancer Cells to Compound Fuling Granule by Protecting Cell Growth, Invasion, Migration, and Stemness. Frontiers in Pharmacology, 11, 150.

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CD63 Expression in LAD2 Cells

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LAD2 cells were washed, suspended in advanced Tyrode’s solution (Procell), and stimulated with different concentrations of RASF for 20 min. Thereafter, the cells were washed, fixed with 2% paraformaldehyde and stained with APC anti-human CD117 (BioLegend, 313206, [104D2], 1:100) and PE anti-human CD63 (Thermo Scientific, 12-0639-42, [H5C6], 1:100). CD63 surface expression was detected using an LSR Fortessa X20 (BD Biosciences).

Lei Y., Guo X., Luo Y., Niu X., Xi Y., Xiao L., He D., Bian Y., Zhang Y., Wang L., Peng X., Wang Z, & Chen G. (2024). Synovial microenvironment-influenced mast cells promote the progression of rheumatoid arthritis. Nature Communications, 15, 113.

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Isolation and RNA-Seq Analysis of Leukemia Cells

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Peripheral blood was taken under written informed consent from patients during routine blood draws at screening and different timepoints during the first cycle of treatment with SDNX-5613 within the AUGMENT-101 clinical trial (NCT04065399). These studies were conducted in accordance to the Declaration of Helsinki and were approved by an institutional review board at the Dana-Farber Cancer Institute (IRB: #01-206). Peripheral blood mononuclear cells (PBMCs) were subsequently isolated using Ficoll (BD Bioscience) gradient centrifugation, viably frozen, and banked at the Dana-Farber Cancer Institute, Boston, MA. For longitudinal analysis, samples were thawed, washed twice in PBS, and stained with anti-human CD45 (PE) (Biolegend, 304007) and anti-human CD117 (APC) (Biolegend, 313205). CD45-low/CD117+ leukemia cells were FACS sorted (Sony MA900 sorter) and subsequently processed for RNA-Seq (see methods section on RNA-Seq).

Soto-Feliciano Y.M., Sánchez-Rivera F.J., Perner F., Barrows D.W., Kastenhuber E.R., Ho Y.J., Carroll T., Xiong Y., Anand D., Soshnev A.A., Gates L., Beytagh M.C., Cheon D., Gu S., Liu X.S., Krivtsov A.V., Meneses M., de Stanchina E., Stone R.M., Armstrong S.A., Lowe S.W, & Allis C.D. (2022). A Molecular Switch between Mammalian MLL Complexes Dictates Response to Menin–MLL Inhibition. Cancer Discovery, 13(1), 146-169.

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CD45lo/CD117+ Leukemia Cell Isolation

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Peripheral blood was taken under written informed consent from patients during routine blood draws at screening and different timepoints during the first cycle of treatment with SDNX-5613 within the AUGMENT-101 clinical trial (NCT04065399). These studies were conducted in accordance with the Declaration of Helsinki and were approved by an institutional review board (IRB) at the Dana-Farber Cancer Institute (IRB: #01-206). Peripheral blood mononuclear cells were subsequently isolated using Ficoll (BD Biosciences) gradient centrifugation, viably frozen, and banked at the Dana-Farber Cancer Institute. For longitudinal analysis, samples were thawed, washed twice in PBS, and stained with anti-human CD45 (PE; BioLegend, 304007) and anti-human CD117 (APC; BioLegend, 313205). CD45^lo/CD117⁺ leukemia cells were FACS-sorted (Sony MA900 sorter) and subsequently processed for RNA-seq (see Methods section on RNA-seq).

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