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2 protocols using anti pfgfr

1

Immunofluorescence Analysis of Wing Disc

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Third instar wing discs were staged and fixed in 4% paraformaldehyde. Immuno-fluorescence was performed as described(Das et al., 2013 (link)). Antibodies used: anti-pRET[Y905], anti-pJnk, anti-pAkt, anti-SOX2, anti-slug, anti-NCadherin, anti-pEGFR[Y845], anti-pEGFR[Y1068], anti-pFGFR[Y653/654], anti-Rab5, anti-Rab7, anti-Rab9(Cell Signaling), anti-pSRC[Y418] (Invitrogen), anti-dpERK (SIGMA), plus anti-Actin,, anti-E-cadherin, anti-α-Catenin, anti-Rho1, anti-Syntaxin, anti-β-tubulin (Developmental Studies Hybridoma Bank), anti-Actin, anti-GAPDH antibodies (Santa Cruz Biotechnology), anti-Rac1 antibody (BD biosciences), anti-KIF5B(Abcam), anti-EGFR (Julia Cordero), anti-Arp3(William Theurkauf).
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2

Anticancer Compounds Characterization

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Erlotinib was kindly provided by F. Hoffman-La Roche Ltd, gefitinib was provided by AstraZeneca Inc; VS-4718 was provided by Verastem Inc; afatinib, osimertinib, lapatinib, AZD4547, and BIBF1120 were purchased from Selleck Chemicals; SU11274 and picropodophyllin were from Carbiochem; dasatinib was from Bio Vision; SB203580 was from Cayman Chemical; sorafenib was acquired from Toronto Research Chemicals Inc, cisplatin was from Bristol-Myers Squibb Company; and PD173074 was from Sigma-Aldrich.
Anti-HER2 and anti-pHER2 antibodies were purchased from Merck Millipore Corporation, anti-EGFR, anti-pEGFR, anti-pHER3, anti-HER4, anti-pHER4, anti-pc-Met, anti-IGF1Rβ, anti-pIGF1Rβ, anti-PDGFRβ, anti-pPDGFRβ, anti-FGFR1, anti-pFGFR, anti-ERK1/2, anti-pERK1/2, anti-AKT, anti-pAKT, anti-STAT3, anti-pSTAT3, anti-PTEN, anti-SRC, anti-FYN, anti-LYN, anti-YES, anti-LCK, anti-pSRC family (Y416), anti-pSRC (Y527), anti-FAK, anti-pFAK (Y397), anti-pFAK (Y576/577), anti-pFAK (Y925) and anti- EGFR (del E746-A750) antibody were from Cell Signaling Technology, anti-HER3 and anti-c-Met were from Santa Cruz Biotechnology Inc, anti-β-actin was from Abcam, Inc., and anti-α-tubulin was from Sigma-Aldrich.
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