The following primary antibodies and the corresponding isotype controls were purchased from BD Pharmingen, Inc. (San Diego, CA, USA): PE‐conjugated anti‐mouse antibodies against CD3, CD11b, CD80, CD206 (clone M1), major histocompatibility complex class II (MHC II), NK1.1, programmed death‐ligand 1 (PD‐L1); FITC‐conjugated anti‐mouse antibodies against CD4, CD45; APC‐conjugated anti‐mouse antibodies against CD8, F4/80; APC‐Cy7‐conjugated anti‐mouse CD11c; PE‐Cy7‐conjugated anti‐mouse CD86; and PerCP‐conjugated anti‐mouse CD40. Anti‐ programmed cell death protein 1 (PD‐1) antibody (clone RPM1‐14) was purchased from Bio X Cell (Lebanon, NH, USA). Functional anti‐CD3 and anti‐CD28 monoclonal antibodies were obtained from Invitrogen (Carlsbad, CA, USA). Penicillin and streptomycin, the DCFH‐DA probe, Alexa Fluor® 488‐labeled goat anti‐rabbit IgG (H+L), Alexa Fluor® 555‐labeled donkey anti‐mouse IgG (H+L), and HRP‐conjugated goat anti‐mouse IgG (H+L) were obtained from Beyotime (Haimen, Jiangsu, China). PX‐478 and GM6001 were obtained from ApexBio (Houston, TX, USA). Matrigel and macrophage colony‐stimulating factor (MCSF) were purchased from Sigma (St. Louis, MO, USA) and GenScript (Nanjing, Jiangsu, China), respectively.
Pe conjugated anti mouse antibodies
Manufactured by BD
Sourced in United States
PE-conjugated anti-mouse antibodies are laboratory reagents used to detect and quantify mouse proteins in various assays, such as flow cytometry and immunohistochemistry. They bind specifically to mouse antibodies or antigens and are labeled with the fluorescent dye phycoerythrin (PE) for visualization and detection purposes.
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Lab products found in correlation
Alexa fluor 555 labeled donkey anti mouse igg h l, by Beyotime (1 mentions)
Px 478, by Apexbio (1 mentions)
Percp conjugated anti mouse cd40, by BD (1 mentions)
Pe cy7 conjugated anti mouse cd86, by BD (1 mentions)
Hrp conjugated goat anti mouse igg h l, by Beyotime (1 mentions)
Alexa fluor 488 labeled goat anti rabbit igg h l, by Beyotime (1 mentions)
Penicillin and streptomycin, by Beyotime (1 mentions)
Cellquest pro, by BD (1 mentions)
2 protocols using pe conjugated anti mouse antibodies
1
Multiparameter Flow Cytometry Analysis
2
Flow Cytometry Analysis of Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After the rst passage, we had harvested the adherent cells from nonunion tissues. The cell-surface protein expressions of NCs were evaluated using the method of ow cytometry. In order to make a comparison with BMSCs, BMSCs were kindly provided by our hospital laboratory and were cultured in the same medium like NCs' conditions. The cells were washed with PBS-3% FBS for several times, and then were incubated with phycoerythrin (PE)-conjugated anti-mouse antibodies for 30 minutes at 4℃ in the dark, which includes CD29, CD34, CD44, and CD45 (BD Biosciences, San Jose, CA, USA). We applied the nonspeci c mouse PE-conjugated IgG (BD Biosciences) as an isotype control. After the incubation, we have washed the cells with PBS-3% FBS twice, and applied the facsaria ow cytometry system (BD Biosciences) and CellQuest Pro (BD Biosciences) for data analyses. The positive staining of cells was scored as literature reported [19] . At least 10000 list mode events were collected for each sample.
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