45.1 transgenic mice 002014

Male or female Il4^–/– (002253) and Il10^–/– (002251), 45.1 transgenic mice (002014), C57BL/6 mice (000664), male and female ΔdblGATA (005653), and Balb/c (000651) mice aged 7–8 weeks were purchased from the Jackson laboratory (Bar Harbor, ME). We previously reported the Il13^–/– mice^{50 (link)}. eoCRE and EOS-less iPHIL mice were described previously^{17 (link)}. eoCRE mice were crossed with a (flox-stop-flox)-GFP reporter strain (B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J, 007906) purchased from the Jackson laboratory. All mice were housed in a pathogen-free facility on a 12 light/12 dark cycle at temperatures of 65–75 °F with 40–60% humidity. All animal procedures conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health, and were approved by the Brigham and Women’s Hospital Standing Committee on Animals (protocol #2016N000442).

C57BL/6 WT mice (000664), Il5^−/− mice (003175), and 45.1 transgenic mice (002014) aged 7–8 weeks were purchased from the Jackson laboratory (Bar Harbor, ME). The Group 2 innate lymphoid cells (ILC2)-deficient mice Rora^fl/flIl7r^Cre/+(carrying a Rora deletion in Il7ra-expressing lymphocytes that leads to ILC2 deficiency in the absence of neurological defects) and ICOS-T (carrying a loxP-flanked diphtheria toxin receptor (DTR) gene inserted into the ICOS locus, which is expressed ‘preferentially’ on T cells and ILC2 cells, that enables CD4^Cre/+–mediated excision of the DTR gene from T cells and its retention in ILC2 cells, enabling ILC2 depletion through DTX and sparing of T cells) were previously reported.^{16 (link)} Mice were used for experiments at age 8–10 weeks old. Mice were anesthetized with isoflurane inhalation and received one dose of meloxicam by subcutaneous injection (3 mg/kg) before surgery. After surgery, mice received subcutaneous meloxicam (3 mg/kg) at one dose each 24 h for 48 h and subcutaneous buprenorphine (0.05 mg/kg) at one dose per 12 h for 48 h. All animal procedures conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health and was approved by the Brigham and Women’s Hospital Standing Committee on Animals (protocol #2016N000442).