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Rabbit anti nd1

Manufactured by Abcam
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Rabbit anti-ND1 is a primary antibody directed against the NADH-ubiquinone oxidoreductase subunit 1 (ND1) protein. ND1 is a subunit of the mitochondrial respiratory complex I. This antibody can be used to detect and study the ND1 protein in various applications.

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Lab products found in correlation

Mouse anti β actin, by Cell Signaling Technology (2 mentions) Rabbit anti hif 1α, by Novus Biologicals (2 mentions) Easypack mini protease inhibitor cocktail, by Roche (2 mentions) Mouse anti sdha, by Abcam (2 mentions) Rabbit anti phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Rabbit anti ampkα 23a3, by Cell Signaling Technology (2 mentions) Mouse anti cox1, by Abcam (2 mentions) Pierce ripa buffer, by Thermo Fisher Scientific (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Phosstop phosphatase inhibitor, by Roche (2 mentions)

2 protocols using rabbit anti nd1

1

Immunoblotting Analysis of Cellular Proteins

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Whole-cell lysates were prepared using lysis buffer (Pierce™ RIPA buffer, Thermo Scientific) supplemented with complete EASYpack Mini Protease Inhibitor Cocktail and PhosSTOP Phosphatase Inhibitor (both from Roche Applied Science). Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling: mouse anti-β-actin, goat-anti-rabbit (Horseradish peroxidase-conjugated), and anti-mouse (Horseradish peroxidase-conjugated). The following antibodies were purchased from Abcam: mouse anti-Sdha, rabbit anti-ND1, and mouse anti-COX1. Rabbit anti-AMPKα (23A3) and rabbit anti-phospho-AMPKα (Thr172) were purchased from Cell Signaling Technology, and rabbit anti-HIF-1α was from Novus biological.
Almeida L., Dhillon-LaBrooy A., Castro C.N., Adossa N., Carriche G.M., Guderian M., Lippens S., Dennerlein S., Hesse C., Lambrecht B.N., Berod L., Schauser L., Blazar B.R., Kalesse M., Müller R., Moita L.F, & Sparwasser T. (2021). Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis. Immunity, 54(1), 68-83.e6.
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2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates were prepared using lysis buffer (Pierce™ RIPA buffer, Thermo Scientific) supplemented with complete EASYpack Mini Protease Inhibitor Cocktail and PhosSTOP Phosphatase Inhibitor (both from Roche Applied Science). Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling: mouse anti-β-actin, goat-anti-rabbit (Horseradish peroxidase-conjugated), and anti-mouse (Horseradish peroxidase-conjugated). The following antibodies were purchased from Abcam: mouse anti-Sdha, rabbit anti-ND1, and mouse anti-COX1. Rabbit anti-AMPKα (23A3) and rabbit anti-phospho-AMPKα (Thr172) were purchased from Cell Signaling Technology, and rabbit anti-HIF-1α was from Novus biological.
Almeida L., Dhillon-LaBrooy A., Castro C.N., Adossa N., Carriche G.M., Guderian M., Lippens S., Dennerlein S., Hesse C., Lambrecht B.N., Berod L., Schauser L., Blazar B.R., Kalesse M., Müller R., Moita L.F, & Sparwasser T. (2021). Ribosome-Targeting Antibiotics Impair T Cell Effector Function and Ameliorate Autoimmunity by Blocking Mitochondrial Protein Synthesis. Immunity, 54(1), 68-83.e6.
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