12 000 series

HPLC (Agilent 12,000 series) detected the phenol degradation in MSM by three new bacterial strains. The separation column was C18 column (Eclipse Plus, 4.6 mm × 100 mm i.d., 3.5 m), and the mobile phase was composed of 0.01% formic acid in water (A) and 0.01% formic acid in acetonitrile (B) with a flow rate of 1 mL/min. The mobile phase was sequentially programmed as follows: 0 min (85% A); 0–5 min (50% A); 5–6 min (10% A); 6–7 min (10% A); 7–7.5 min (85% A); and 7.5–10 min (85% A). The standard solutions and samples were injected at a volume of 40 µL, the column temperature was kept at room temperature, and the phenol degradation rate was monitored at 280 nm [76 (link)]. The elutes were identified by comparing their retention time to the reference compound.

Fructose concentration was measured by HPLC (12000 Series, Agilent Technologies, Palo Alto, CA, USA) using a Shodex Asahipak NH2P-50 4E column (4.6 mm ID × 250 mm; Shodex, Kanagawa, Japan). The fructose were detected with a refractive index detector (RID; 1200, Agilent Technologies, Palo Alto, CA, USA) at 30 °C with a flow rate 1 mL/min of a mixture of acetonitrile (J.T. Baker Chemical Co. Phillipsburg, NJ, USA) and water (H2O/CH3CN = 40/60) as a mobile phase.