Cmp mas 4 mm 1h 13c 19f 2h probe

The ¹H NMR experiments were performed on a Bruker Avance III 500 MHz spectrometer, using a prototype CMP MAS 4 mm ¹H–¹³C–¹⁹F–²H probe fitted with an actively shielded Z gradient (Bruker BioSpin) at a spinning speed of 1000 Hz. The oocyte was placed into a 4 mm o.d. zirconium rotor with 10 μl D₂O and the experiments were locked using D₂O solvent. Water suppression was achieved using the purge pulse sequence.34 (link) All spectra were recorded with 256 scans, recycle delay set at 5 × T₁ and ∼4 μs 90° pulse widths for the blank and injected oocyte experiment respectively. 32 768 time domain points were acquired for each spectrum with a spectral width of 20 ppm. Data were zero filled and multiplied by an exponential window function corresponding to a 1 Hz line broadening in the transformed spectrum.

The ¹H NMR experiments were performed on a Bruker Avance III 500 MHz spectrometer, using a prototype CMP MAS 4 mm ¹H–¹³C–¹⁹F–²H probe fitted with an actively shielded Z gradient (Bruker BioSpin) at 37 °C. The samples were all spun at a spinning speed of 6666 Hz and all experiments were locked using D₂O solvent. Water suppression was achieved using water suppression by gradient-tailored excitation (WATERGATE) and was carried out using a W5 pulse train.35 (link) All spectra were recorded with 256 scans, recycle delay set at 5 × T₁, 5.8 μs 90° pulse widths and collected using 32 768 time domain points with spectral widths of 20 ppm. Data were zero filled and multiplied by an exponential window function corresponding to a 1 Hz line broadening in the transformed spectrum.