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Mouse monoclonal antibody against actin

Manufactured by Merck Group

Mouse monoclonal antibody against actin. This product is a laboratory reagent used for the detection and quantification of actin, a cytoskeletal protein, in various biological samples.

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2 protocols using mouse monoclonal antibody against actin

1

AMFR Protein Expression and Regulation

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Primers, Dynabeads protein A, Superscript VILO cDNA synthesis kit, VCP siRNA, and SVIP siRNA were purchased from Life Technologies (Carlsbad, CA), and X-tremeGENE siRNA and, HP DNA transfection reagent were purchased from Roche Applied Science (Indianapolis, IN). The rabbit polyclonal antibodies against AMFR and ubiquitin and anti-rabbit IgG (conformation specific) were purchased from Cell Signaling (Danvers, MA). The rabbit polyclonal antibody against N-terminus AMFR was purchased from LifeSpan BioScience (Seattle, WA). Polyclonal anti-rabbit antibody against SVIP and mouse monoclonal antibody against actin were purchased from Sigma-Aldrich (St. Louis, MO) Polyclonal anti-rabbit antibodies against p97/VCP and IP lysis buffer were purchased from Thermo Fisher scientific (Waltham, MA). Polyclonal anti-rabbit antibody against AAT was purchased from Dako (Carpentaria, CA).
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2

Emodin Molecular Mechanisms Investigation

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Emodin (purity >98%) was purchased from Calbiochem Inc (San Diego, CA) and was dissolved in dimethyl sulfoxide (DMSO; Sigma, Shanghai, China). Antibodies against c-ABL (C-19), PI3K, AKT, and PTEN were from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse monoclonal antibody against actin was from Sigma-Aldrich (St Louis, MO). Anti-mouse immunoglobulin G and anti-rabbit immunoglobulin G horseradish peroxidase-conjugated antibodies were from Pierce Biotechnology (Rockford, IL). Trizol reagent was from Invitrogen (Carlsbad, CA).
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