Urine samples were collected and registered in the DHP and their insulin and lactate content was quantified using a Mesoscale Discovery Human Insulin Kit and a fluorescence based assay, respectively, as described previously [11 (link),36 (link)].
Human insulin kit
Manufactured by Mesoscale
Sourced in United States
The Human Insulin Kit is a laboratory instrument designed for the quantitative detection and measurement of human insulin levels. It provides a reliable and accurate method for researchers to assess insulin concentrations in various biological samples.
Automatically generated - may contain errors
3 protocols using human insulin kit
1
Urine-based Insulin and Lactate Quantification
2
Dietary Intake and Metabolic Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dietary intake was evaluated using a three-day food journal along with a personal interview by the clinical researcher (N.S.). The weight of each consumed product was either provided by the participant or estimated using average quantities per portion. Macro- and micronutrient composition was determined based on the Dutch food composition table [28 ]. Fructose intake was calculated with an extensive sugar composition table, as described previously [11 (link),29 (link)].
Height was determined standing upright against a stadiometer and weight was assessed in solely underwear using an electronic scale. BMI was calculated as weight (kg) divided by height (m) squared. Waist was measured with a measuring tape at the level of the umbilicus.
Blood was drawn for measurement of whole blood glucose (YSI, Yellow Springs, OH, USA), plasma insulin (Human insulin kit, Meso Scale Discovery, Rockville, MD, USA), and serum lipids (Enzymatic colorimetric assay, Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany).
Urinary sodium concentration (Indirect ISE-method, Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany) was determined in the twenty-four-hour urine samples.
Height was determined standing upright against a stadiometer and weight was assessed in solely underwear using an electronic scale. BMI was calculated as weight (kg) divided by height (m) squared. Waist was measured with a measuring tape at the level of the umbilicus.
Blood was drawn for measurement of whole blood glucose (YSI, Yellow Springs, OH, USA), plasma insulin (Human insulin kit, Meso Scale Discovery, Rockville, MD, USA), and serum lipids (Enzymatic colorimetric assay, Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany).
Urinary sodium concentration (Indirect ISE-method, Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany) was determined in the twenty-four-hour urine samples.
3
Plasma Biomarker Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected in tubes containing EDTA and centrifuged at 1,000g for 10 min at 4 °C. Aliquots of plasma were frozen in liquid nitrogen and stored at -80 °C. Plasma glucose and insulin concentrations were analyzed using commercially available kits (ABX Pentra Glucose HK CP, Horiba ABX, ref:
A11A01667 and Human Insulin kit, Meso Scale Discovery, ref: K151BZC). Quantification of plasma AA concentrations was performed using ultra-performance liquid chromatography mass spectrometry (ACQUITY UPLC H-Class with QDa; Waters), as described previously (Nyakayiru et al., 2019) (link).
A11A01667 and Human Insulin kit, Meso Scale Discovery, ref: K151BZC). Quantification of plasma AA concentrations was performed using ultra-performance liquid chromatography mass spectrometry (ACQUITY UPLC H-Class with QDa; Waters), as described previously (Nyakayiru et al., 2019) (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!