Cells were labeled for 3 h with BrdU (Roche), followed by fixation in 70% ethanol. After incubation with 0.1 mg/mL RNase A (Roche) and denaturation in 2 M HCL/0.2% Triton X-100, immunostaining for BrdU was performed using anti-BrdU antibody (DAKO) and FITC-conjugated anti-mouse antibody (DAKO). DNA was stained with propidium iodide. Samples were analyzed for fluorescein and propidium iodide on a BD Biosciences FACSCalibur flow cytometer. SA-β-galactosidase was detected using the Senescence Associated β-galactosidase staining kit (Cell Signaling) at pH6, following the manufacturer's instructions.
Fitc conjugated anti mouse antibody
Manufactured by Agilent Technologies
Sourced in Denmark
The FITC-conjugated anti-mouse antibody is a laboratory reagent used for the detection and identification of mouse proteins in various analytical techniques. It is a fluorescently labeled antibody that specifically binds to mouse immunoglobulins, allowing for the visualization and quantification of target molecules in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (1 mentions)
Bromodeoxyuridine (brdu), by Roche (1 mentions)
Anti brdu antibody, by Agilent Technologies (1 mentions)
Rnase a, by Roche (1 mentions)
Senescence associated β galactosidase staining kit, by Cell Signaling Technology (1 mentions)
Facscanto 2, by BD (1 mentions)
2 protocols using fitc conjugated anti mouse antibody
1
Cellular Senescence Measurement by Flow Cytometry
2
Antibody-Dependent Enhancement Assay for DENV-2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Heat inactivated mice sera were diluted 1:320 (based on initial titration, data not shown) and incubated with DENV-2 (isolate 3229/11) with a multiplicity of infection of 0.1 for 1 h at 37 °C. Then, these mixtures were added to K952 cells and incubated for 2 h. A wash step was applied by adding RPMI 1640 medium with 10% FCS, 2 mM Glutamine and 1% penicillin/streptomycin and subsequent centrifugation. After 2 days, the cells were analyzed by flow cytometry (FACSCanto II, Becton Dickinson, Franklin Lakes, NJ, USA) using the flavivirus-specific 4G2 antibody (1:500) and a FITC-conjugated anti mouse antibody for detection (Dako, Glostrup, Denmark, 1:50). The n-fold increase of enhancement was calculated as: (numbers of positive cells/numbers of all cells in the presence of immunized mouse sera)/(numbers of positive cells/numbers of all cells in the presence of control mouse sera) [29 (link)]. Tests were performed in two independent experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!