Agilent one color microarray

Differential gene expression of untreated HeLa cells and HeLa cells treated with FKB for 48 hours was assayed by Agilent one color microarray using a human gene expression v2 microarray chip (Agilent Technologies, USA). In brief, RNA was extracted from control and FKB-treated HeLa cells by Qiagen RNeasy plus mini kit (Qiagen, USA). Then, the quantity and quality of extracted RNA were tested by nano-drop spectrometer (Eppendorf, USA) and 2100 Bioanalyzer with RNA pico chip (Agilent Technologies, USA). Three biological replicates of control and treated HeLa cell RNA with bioanalyzer RIN number >9 were subjected to microarray using SurePrint G3 Human Gene Expression 8 × 60K v2 microarray kit (Agilent Technologies, USA) according to the manufacturer’s protocol and scanned with Agilent DNA microarray scanner. Differential expression comparison between untreated controls and HeLa cells treated with FKB for 48 hours was analyzed by GeneSpring GX (Agilent Technologies, USA). Genes based on gene ontology with expression level and with significant changes as determined by Student’s t-test (P< .05) and fold changes >2 are presented in this study. The results from this microarray study can be accessed via NCBI GEO using the accession number GSE72974.