Chromid esbl id

Rectal swabs (Copan eSwab including 1 mL of modified liquid Amies) were analysed for the presence of HR-GNRs at the RPHLK by direct culturing on both an ESBL screening agar (ChromID ESBL-ID, bioMerieux, enriched with a mixture of antibiotics, including cefpodoxime) and a CLED GM20 agar (with 20 mg/L gentamicin, Oxoid) and incubated overnight at 37°C. Gram-negative rods growing on these two agars were identified using MALDI-TOF (Bruker Daltonics, Germany). Antibiotic susceptibility testing was performed using the automated system VITEK2 (bioMérieux, France) using CLSI breakpoints. All isolates suspected for the production of ESBL, defined as a VITEK 2 AES alert and/or elevated MIC (> 1 mg/L) for cefotaxime and/or ceftazidime were confirmed using the combination disk method (ceftazidime and cefotaxime or cefepime with and without clavulanic acid) [12 ]. Bacteria with a VITEK 2 AES alert and/or elevated MIC for meropenem (> 0.25 mg/L) were suspected for carbapenemase production and confirmed using the modified Hodge test [12 ]. All positive isolates were stored at -80°C. Molecular characterization of baseline HR-GNR isolates was performed by Whole Genome Sequencing (WGS) based on earlier described methods [13 –16 (link)].

Rectal swabs (Copan eSwab including 1 mL of modified liquid amies) were analyzed for the presence of HR-GNRs at the RPHLK by direct culturing on both an ESBL screening agar (ChromID ESBL-ID, bioMerieux, enriched with a mixture of antibiotics, including cefpodoxime) and a CLED GM20 agar (with 20 mg/L gentamicin, Oxoid). Gram-negative rods growing on these two agars were identified using MALDI-TOF (Bruker Daltonics, Germany). Antibiotic susceptibility testing was performed using the automated system VITEK2 (bioMérieux, France). All isolates suspected for the production of ESBL were confirmed using the combination disk method (ceftazidime and cefotaxime or cefepime with and without clavulanic acid) [16 ]. Strains suspected for carbapenemase production were confirmed using the modified Hodge test [16 ]. All positive isolates were stored at −80 °C.