Allprep rna dna

Manufactured by Qiagen

The AllPrep RNA/DNA Kit is a laboratory product that allows for the simultaneous purification of both RNA and DNA from a single sample. It is designed to provide high-quality, purified nucleic acids for various downstream applications.

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Lab products found in correlation

Vilo cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Allprep dna rna micro kit, by Qiagen (2 mentions) Taqman fast advanced master mix, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

AllPrep PowerFecal DNA/RNA kit AllPrep PowerViral DNA/RNA Kit Allprep DNA RNA mini prep kit AllPrep DNA/RNA procedure Allprep DNA/RNA FFPE tissue kit AllPrep RNA/DNA minikits AllPrep DNA/RNA isolation kit QIAGEN AllPrep DNA/RNA Mini Kit RNeasy kit AllPrep DNA/RNA Nano Kit Allprep DNA/RNA Plus Micro Kit

2 protocols using allprep rna dna

Transcriptional Profiling of MAIT Cells

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MAIT cells (CD3⁺ CD44⁺ TCRβ⁺ MR1-5-OP-RU tetramer⁺) and non-MAIT (MR1-5-OP-RU tetramer^- TCRβ⁺) CD3⁺ T cell populations were sorted into 350 µL RLT plus buffer from the Qiagen Allprep DNA/RNA micro kit. Nucleic acid extraction was performed according to manufacturer’s instructions (Qiagen Allprep RNA/DNA). cDNA was synthesized using SuperScript VILO cDNA Synthesis Kit and qRT-PCR analysis on Ifng, Il17a, Gzmb, and Prf1 genes was performed with specific TaqMan primers and TaqMan Fast Advanced Master Mix (Applied Biosystems). The mRNA levels of specific genes were determined by the relative standard curve method, normalized against housekeeping ribosomal protein L32 levels. ΔCT was calculated as CT_gene – CT_housekeeping and ΔΔCT was calculated as ΔCT_CLP – ΔCT_sham.

Trivedi S., Labuz D., Anderson C.P., Araujo C.V., Blair A., Middleton E.A., Jensen O., Tran A., Mulvey M.A., Campbell R.A., Hale J.S., Rondina M.T, & Leung D.T. (2020). Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis. eLife, 9, e55615.

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Profiling MAIT Cell Gene Expression

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MAIT cells (CD3 + CD44 + TCRβ + MR1-5-OP-RU tetramer + ) and non-MAIT (MR1-5-OP-RU tetramer -TCRβ + ) CD3 + T cell populations were sorted into 350 µl RLT plus buffer from the Qiagen Allprep DNA/RNA micro kit. Nucleic acid extraction was performed according to manufacturer's instructions (Qiagen Allprep RNA/DNA). cDNA was synthesized using SuperScript VILO cDNA Synthesis Kit and qRT-PCR analysis on Ifng, Il17a, Gzmb and Prf1 genes was performed with specific TaqMan primers and TaqMan Fast Advanced Master Mix (Applied Biosystems). The mRNA levels of specific genes were determined by the relative standard curve method, normalized against housekeeping ribosomal protein L32 levels. ΔCT was calculated as CTgene -CThousekeeping and ΔΔCT was calculated as ΔCTCLP -ΔCTsham.

Trivedi S., Labuz D., Anderson C., Araujo C.V.d., Blair A., Middleton E.A., Tran A., Mulvey M.A., Campbell R.A., Hale J.S., Rondina M.T., & Leung D.T. (2020). Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis.

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