Mem medium without l glutamine

EGCG, 4-OH-Hydroxy-tamoxifen, L-glutamine, Penicillin-Streptomycin, Sulforhodamine B, MEM medium without L-glutamine and phenol red, phytohaemagglutinin (PHA), 4′,6-diamidino-2-phenylindole (DAPI), 1,4-Diazabicyclo[2.2.2]octane (DABCO) and G418 were all purchased by Sigma-Aldrich, MO, USA. E-MEM, D-MEM, RPMI 1640, and FBS were purchased by Lonza Group Ltd., Basel, Switzerland. Formalin 40% was from Carlo Erba, Milano, Italy. The following antibodies were used: anti-Bax from Applied Biosystem, USA; anti-Bcl2, anti-LR67, anti-actin, anti-γ-tubulin and anti-mouse-FITC conjugated from Sigma-Aldrich, MO, USA; anti-AKT, anti-p473AKT, anti-Foxo3a, anti-rabbit and anti-mouse-IgG-HRP-conjugated from Thermo Scientific, IL, USA; anti rabbit-IgG-Rhodamine-conjugated from Novus Biologicals, CO, USA. Lipofectamine 2000 was purchased by Invitrogen, CA, USA.

EGCG, 4-Hydroxytamoxifen, L-glutamine, penicillin-streptomycin, sulphorhodamine B, MEM medium without L-glutamine and Phenol Red, DAPI and 1,4-diazabicyclo[2.2.2]octane (DABCO), were all purchased from Sigma–Aldrich, MO, U.S.A. IIF (pat. WIPO W0 00/17143), was provided by Dr K. Ammar, Bologna, Italy. E-MEM, D-MEM and FBS were purchased from the Lonza group, Basel, Switzerland. Formalin 40% was from Carlo Erba, Milano, Italy. Antibodies: anti-EGFR, anti-AKT and anti-p473AKT, were all from Thermo Scientific, Waltham, MA, U.S.A., anti-p1068EGFR from Novex, Life Technologies, Carlsbad, CA, U.S.A., anti-mouse-FITC conjugated and anti-γ-tubulin (Sigma–Aldrich, MO, U.S.A.), anti-p308AKT (Rockland Immunochemicals, Pottstown, PA, U.S.A.), anti-CD44 (BD Bioscience, San Jose, CA, U.S.A.), anti-EMMPRIN (Zymed Lab, San Francisco, CA, U.S.A.), anti-MMP-2, MMP-9 and anti-TIMPs (all from Santa Cruz, Dallas, TX, U.S.A.), anti-rabbit and anti-mouse-peroxidase conjugated antibodies (GE Healthcare, Milano, Italy).