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Rna purified total rna extraction kit rp1201

Manufactured by BioTeke
Sourced in China

The RNA Purified Total RNA Extraction Kit (RP1201) is a laboratory tool designed for the extraction and purification of total RNA from various biological samples. The kit utilizes a column-based method to isolate high-quality RNA that can be used for downstream applications such as reverse transcription, quantitative PCR, and other RNA analysis techniques.

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2 protocols using rna purified total rna extraction kit rp1201

1

Investigating NF-κB Signaling Pathway

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Antibodies against Fn14 (D121267) and Src (D120536) were purchased from Sango Biotech (Shanghai, China). Antibodies against IκBα (bs-1287R), phosphorylated IκBα (p-IκBα) (bs-18129R), IKKβ (bs-4880R), p-IKKβ (bs-3233R), and Histone H3 (bs-17422R) were purchased from Bioss (Beijing, China). Antibody against nuclear NF-κB p65 (PB0073) was purchased from Boster (Wuhan, China). Antibody against NF-κB p65 (10745-1-AP) used for immunofluorescent staining was purchased from Proteintech (China). Secondary goat anti-rabbit IgG-HRP (A0208), goat anti-mouse IgG-HRP (A0216), and cy3-labled goat anti-mouse IgG (A0521) antibodies were purchased from Beyotime (Shanghai, China). Antibody against β-actin (sc-47778) was purchased from Santa Cruz (CA, USA). RNA Purified Total RNA Extraction Kit (RP1201) and super M-MLV reverse transcriptase (RP6502) were purchased from BioTeke (Beijing, China). Total Protein Extraction Kit (P0027) was purchased from Beyotime (Shanghai, China). MTT (M-2128) was purchased from Sigma (USA).
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2

Berberine Biomarker Detection Protocol

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Berberine was purchased from MedChemExpress (Monmouth Junction, NJ) and dissolved in saline. Agents for PCR detection, including RNA Purified Total RNA Extraction Kit (RP1201) and super M-MLV reverse transcriptase (RP6502) were purchased from BioTeke (Beijing, China). Agents for western blotting detection, including PMSF lysis (P0013B), protein concentration determination kit (P0012), cell cycle detection kit (C1052), CCK-8 detection kit (C0037) and Hoechst staining kit (C0003) were purchased from Beyotime Biotechnology (Shanghai, China). Cell apoptosis detection kit (KGA106) was purchased from KeyGen BioTECH (Nanjing, China). Detection kits for malondialdehyde (MDA) (A003-1), glutathione (GSH) (A006-2), glutathione peroxidase (GSH-Px) (A005) and superoxide dismutase (SOD) (A001-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Detection kits for tumour necrosis factor-α (TNF-α) (EK3821/2), interleukin-1β (IL-1β) (EK301B1/2) and IL-6 (70-EK206) were purchased from Multi Sciences (Hangzhou, China).
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