Log In Sign up
The largest database of trusted experimental protocols

Phosphoric acid

Manufactured by Chimmed
Visit Supplier

Phosphoric acid is a colorless, odorless, and highly corrosive inorganic acid. It is a key component in the production of various chemical compounds and is widely used in industrial processes.

Automatically generated - may contain errors

Lab products found in correlation

Ethanol, by Chimmed (2 mentions) Bovine igg, by Reanal (2 mentions) 96 well plate, by Corning (2 mentions) Infinite m200 pro microplate reader, by Tecan (1 mentions) Infinite m200 pro plate spectrophotometer, by Tecan (1 mentions)

2 protocols using phosphoric acid

1

Quantitative Protein Determination by Microplate

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was assayed after Bradford with modifications24 (link),25 (link) using a 96-well plate (Corning, United States) and a UV/VIS Infinite M200 PRO microplate reader (Tecan, Switzerland). The dye solution contained 0.03% Coomassie G-250 (LOBA Feinchemie, Austria), 5% ethanol, and 10% phosphoric acid (Chimmed, Russia). Bovine IgG (Reanal, Hungary) was used for the calibration curve.
Selina P.I., Karaseva M.A., Komissarov A.A., Safina D.R., Lunina N.A., Roschina M.P., Sverdlov E.D., Demidyuk I.V, & Kostrov S.V. (2021). Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos. Scientific Reports, 11, 18196.
+ Open protocol
+ Expand
2

Protein Quantification by Bradford Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was assayed in cell lysates after Bradford with modifications [27 (link), 28 (link)] using 96-well plates (Corning, Corning, New York, USA) and an Infinite M200 PRO plate spectrophotometer (Tecan, Männedorf, Zürich, Switzerland). The staining solution contained 0.03% Coomassie G-250 (LOBA Feinchemie, Fischamend, Austria), 5% of ethanol, and 10% of phosphoric acid (Chimmed, Moscow, Russia). The calibration curve was plotted using bovine IgG (Reanal, Budapest, Hungary) as the standard.
Safina D.R., Selina P.I., Roschina M.P., Karaseva M.A., Komissarov A.A., Demidyuk I.V., Sverdlov E.D, & Kostrov S.V. (2020). Functional efficiency of PCR vectors in vitro and at the organism level. PLoS ONE, 15(4), e0232045.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!