Ivd matrix hcca portioned

Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) (Microflex LT/SH, Bruker, Germany) and IVD-Database Knowledge Version 8 (7854MSP, Bruker, Germany) were used to identify the bacterial strain. The strain Shenzhen WH 0221 was cultivated anaerobically on Columbia Blood Agar at 37°C for 1 day. Fresh bacterium was spotted on a target plate (96 polished ID101, Bruker, Germany) and covered first with 1 μL 70% formic acid, then with 1 μL IVD Matrix HCCA-portioned (Bruker, Germany). The sample was analyzed using the MALDI-TOF MS instrument following the manufacturer’s recommendations. Positive identification was defined as a confidence value ≥2.0.

Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) (Microflex LT/SH, Bruker, Germany) and IVD-Database Knowledge Version 8 (7854MSP, Bruker, Germany) were used to identify the bacterial strain. The strain Shenzhen WH 0221 was cultivated anaerobically on Columbia Blood Agar at 37°C for 1 day. Fresh bacterium was spotted on a target plate (96 polished ID101, Bruker, Germany) and covered first with 1 μL 70% formic acid, then with 1 μL IVD Matrix HCCA-portioned (Bruker, Germany). The sample was analyzed using the MALDI-TOF MS instrument following the manufacturer’s recommendations. Positive identification was defined as a confidence value ≥2.0.

The exact identification of the bacteria was carried out using MALDI-TOF microflexTM LT/SH (Bruker AXS GmbH, Billerica, MA, USA) at the Institute for Laboratory Diagnostics and Microbiology of the Klinikum Klagenfurt am Wörthersee, Austria. The matrix used was the IVD matrix HCCA-portioned from Bruker.