Nqo1 primary antibody

3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) was obtained from AccuStandard Inc. (New Haven, CT). VCAM-1 and Keap1 primary antibodies and horseradish peroxidase-conjugated goat secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). β-actin and GST primary antibodies were purchased from Sigma (St. Louis, MO). The GST antibody used recognized native as well as denatured-reduced forms of GST protein. NQO1 primary antibody was purchased from Abcam (Cambridge, MA). Fyn kinase, P-Akt, Akt, P-PKC delta and Nrf2 primary antibodies and horseradish peroxidase-conjugated rabbit secondary antibody were purchased from Cell Signaling Technologies (Danvers, MA).

15-oxoeicosatetraenoic acid (15-oxoETE) was purchased from Cayman Biochemical (Ann Arbor, MI). Ethacrynic acid and secondary antibodies were purchased from Santa Cruz Biotechnologies (Dallas, TX). LPS derived from E.coli 0127:B8, phorbol myristoyl acetate (PMA), actin primary antibody, wedelolactone and diethyl ether were purchased from Sigma-Aldrich (St. Louis, MO). Primary HO-1 and the IKKβ Kinase assay kit were purchased from Cell Signaling (Beverly, MA). The NQO1 primary antibody was purchased from Abcam (Cambridge, MA), GCLM from Proteintech (Chicago, IL), and GAPDH from Trevigen (Gaithersburg, MD). Solvents for liquid chromatography mass spectrometry (LC-MS) including pure water and acetonitrile were purchased from Burdick and Jackson (Morristown, NJ). Glacial acetic acid, Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), Optima grade methanol and chloroform were purchased from Fisher Scientific (Pittsburgh, PA). The luciferase reporter assay was purchased from Promega (Madison, WI) and the human recombinant TNFα was purchased from Life technologies (Grand Island, NY).