Dna sodium salt from calf thymus ct dna

Ext.DrewAT, DrewAT, Ds26, misTA, allAT, and allCG30 31 (link) were obtained from Integrated DNA Technologies (Coralville, IA, USA) (Table S2). The autocomplementary oligonucleotides (DrewAT, Ext.DrewAT, Ds26, allAT, and allCG) were heated at 95 °C for 5 min in milliQ water and then slowly cooled to room temperature to generate double-stranded DNA structures. Concentrations were determined by measuring UV-Vis absorption using the supplier’s values for the molar extinction coefficients at 260 nm. DNA sodium salt from calf thymus (CT-DNA) and RNA from torula yeast were purchased from Sigma-Aldrich (St. Louis, MO, USA). CT-DNA and RNA were dissolved in 10 mM sodium phosphate buffer (pH 7.4, 100 mM NaCl) and filtered. Concentrations were evaluated by UV considering that an OD 260 nm of 1 corresponds to approximately 50 μg/mL double-stranded DNA and 40 μg/mL RNA, respectively.