Recombinant plasmids

Aβ42 was synthesized by GL Biochem (Shanghai, China). Recombinant plasmids were synthesized by GenScript (Nanjing, China). BL21(DE3) competent cells were purchased from TransGen Biotech (Beijing, China). 3,3,5,5′-tetramethylbenzidine (TMB) was purchased from Tiangen Biotech (Beijing, China). Nitrocellulose membrane was purchased from Whatman (Maidstone, UK). Non-pre-stained Protein Marker was purchased from Thermo Fisher (Rockford, USA). Horseradish peroxidase-labeled (HRP-labeled) goat anti-human IgG (H + L) was purchased from Sino Biological (Beijing, Chian). Anti-His-tag monoclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Protein Marker was purchased from Bio-Rad (CA, USA). Mouse monoclonal antibody, β-Amyloid (B-4), recognizing Aβ42 was purchased from Santa Cruz Biotechnology, INC.

Recombinant plasmids (GenScript, USA), derived from pET-28a, encoding the full sequence of DENV-2 strain NGC (GenBank reference number M29095) and ZIKV NS1 based on the Brazilian strain (GenBank reference number ALU33341) were introduced into chemically competent E. coli BL21-Codon Plus (DE3)-RIL strain (Stratagene, USA). The plasmid encoding the ZIKV ΔNS1 carries the genetic sequence corresponding to the last 100 amino acids of the C-terminal region of ZIKV NS1 (patent application number: BR 10 2016 011318 0). The plasmid encoding DENV-2 NS1 was previously reported (Amorim et al. 2010 (link)). Recombinant expression plasmids encoding the ΔNS1 were introduced in E. coli Arctic Express (DE3) (Agilent) and BL21 (DE3) (Invitrogen) strains.