Angii inhibitor cocktail and phenyl cartridges

A commercially available sandwich ELISA (R&D systems) was used according to the manufacturer’s guidelines to measure ACE-1 levels in serum. ACE-1 concentrations were determined by interpolation from the standard curve determined for each plate from the known. The concentration of ACE-1 is expressed in ng/ml. The interassay coefficient of variance for ACE-1 measurements was 3%. The assay sensitivity was 0.05 ng/ml. The concentrations of ANGII were measured by enzyme immunoassay using plates coated with ANGII monoclonal antibody and a detection antibody labeled with acetylcholinesterase, and ANGII inhibitor cocktail and phenyl cartridges for sample preparation and extraction following the manufacturer’s protocol (Cayman Chemical). The concentration of ANGII is expressed in pg/ml. The interassay coefficient of variance for ANGII measurements was 6.2%. The assay sensitivity was 2.0 pg/ml. ACE-1 and ANGII measurements were normalized using log-transformations.

A commercially available sandwich ELISA (R&D systems) was used according to the manufacturer’s guidelines to measure ACE-1 levels in serum. ACE-1 concentrations were determined by interpolation from the standard curve determined for each plate from the known. The concentration of ACE-1 is expressed in ng/ml. The interassay coefficient of variance for ACE-1 measurements was 3%. The assay sensitivity was 0.05 ng/ml. The concentrations of ANGII were measured by enzyme immunoassay using plates coated with ANGII monoclonal antibody and a detection antibody labeled with acetylcholinesterase, and ANGII inhibitor cocktail and phenyl cartridges for sample preparation and extraction following the manufacturer’s protocol (Cayman Chemical). The concentration of ANGII is expressed in pg/ml. The interassay coefficient of variance for ANGII measurements was 6.2%. The assay sensitivity was 2.0 pg/ml.