Rutin

Alcian blue, aluminium chloride, Folin-Ciocalteu reagent, ethylene diamine tetra acetic acid (EDTA), 5,5-dithio-bis-(2-nitrobenzoic acid (DTNB), gallic acid, thiobarbituric acid, trichloroacetic acid, toluene, ethyl acetate, formic acid, formaldehyde, sodium hydroxide, rutin, phosphoric acid, pyrogallol, haematoxylin, eosin were purchased from HiMedia Laboratories Pvt Ltd, Mumbai, India, and HPTLC plates, tris-HCl buffer, ethanol, amentoflavone were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA).

α,α_-diphenyl-_-picrylhydrazyl (DPPH) and 2,2_-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) were purchased from Sigma–Aldrich (St. Louis, MO). Ascorbic acid (vitamin C), gallic acid, rutin, nitro blue tetrazolium (NBT), and butylated hydroxytoluene (BHT) were purchased from Hi-Media, India. The remaining chemicals and solvents used were of standard analytical grade and HPLC-grade. 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) and Hoechst 33342 dye were obtained from from Sigma (St. Louis, MO, United States ). DMEM-high glucose medium, fetal bovine serums (FBS), and antibiotic–antimycotic solution were procured from Himedia India, Ltd., Mumbai, India, whereas a colorimetric kit specific for caspase-8, -9, and -3 was provided by BioVision, CA, United States . The DyNAmoColorFlash SYBR Green qPCR Kit (F415L) along with the Verso cDNA synthesis kit were procured from Thermo-Scientific, United States . The primers used in the present work were synthesized and purchased from IDT, United States .

Alcian blue, aluminium chloride, Folin-Ciocalteu reagent, ethylene diamine tetra acetic acid (EDTA), 5,5-dithio-bis-(2-nitrobenzoic acid (DTNB), gallic acid, thiobarbituric acid, trichloroacetic acid, toluene, ethyl acetate, formic acid, formaldehyde, sodium hydroxide, rutin, phosphoric acid, pyrogallol, haematoxylin, eosin were purchased from HiMedia Laboratories Pvt Ltd, Mumbai, India, and HPTLC plates, tris-HCl buffer, ethanol, amentoflavone were purchased from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA).