Mouse anti sars cov 2 spike antibody

The cells were cultured in confocal dishes overnight at 37°C, and then, incubated with a 2% FBS DMEM with 30μl SARS-CoV-2 pseudovirus or a 2% FBS DMEM only for 24 h. Next, the cells were fixed with 4% paraformaldehyde fix solution (AR1069, Boster) for 15 min and permeabilized with 0.02% Triton X-100 (A600198-0500, BBI Life Sciences) for 3 min, followed by incubation with 5% goat serum for 1 h. Subsequently, the cells were incubated with the corresponding primary antibodies (rabbit anti-Arf6 antibody, PA1-093, Invitrogen; rabbit anti-Rab5a antibody, 2143T, cell signaling technology; mouse anti-SARS-CoV-2 spike antibody, Sino Biological; human anti-CD147 antibody, MPZ, Jiangsu Pacific Meinuoke Biopharmaceutical Co. Ltd) overnight at 4°C. After being washed three times with PBS, the cells were stained with corresponding immunofluorescent secondary antibodies (goat anti-human IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 647, A21445, Invitrogen, donkey anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor 488, A21202, Invitrogen, donkey anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor 555, A31572, Invitrogen) for 1 h at room temperature, and DAPI was used to stain the nuclei for 10 min. The images were collected using confocal laser scanning microscopy.