Malt1 ep603y

Equal amounts (20 μg or 40 μg) of the cell extracts were separated on a 10% or 12% dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel. The blotting membranes were probed using an antiserum of ARv7 and ARFL (MA5-13426, Invitrogen, Carlsbad, CA, USA), MALT1 (EP603Y, Abcam, Cambridge, MA, USA), NF-κB p50 (06-886, Merck Millipore, Burlington, MA, USA), NF-κB p65 (06-418, Merck Millipore, Burlington, MA, USA), Lamin B1 (D9V6H, Cell signaling Technology Inc., Danvers, MA, USA), IκB-α (#9242, Cell signaling Technology Inc.), p-IκB-α (#2859, Cell signaling Technology Inc.), GAPDH (6C5, Santa Cruz Biotechnology, Dallas, TX, USA), NDRG1 (42-6200, Thermo Fisher Scientific Inc., Vilnius, Lithuania), PSA (A0562, Dako Denmark A/S, Glostrup, Denmark), or β-actin (T0022, Affinity bioscience, Cincinnati, OH, USA). The band intensities in the blot membrane were detected by the Western Lightning^TM Plus Chemiluminescence detection system (PerkinElmer Inc., Waltham, MD, USA), recorded by the LuminoGraph II chemiluminescent imaging system (Atto Corporation, Tokyo, Japan), and analyzed by the Image J software (version 1.52a).

Equal amounts of cell lysates were separated on 10% or 12% sodium dodecyl sulfate-polyacrylamide gels. The blotting membranes were probed using antiserum of GAPDH (6C5), MALT1 (EP603Y, Abcam, Cambridge, MA, USA), β-actin (MAB1501), NF-κB p50 (06-886), NF-κB p65 (06-418, Merck Millipore, Burlington, MA, USA), Lamin B1 (D9V6H), IκB-α (#9242), p-IκB-α (#2859, Cell Signaling Technology, Inc. Danvers, MA, USA), NDRG1 (42-6200, Thermo Fisher Scientific Inc.), or BTG2 [31 (link)]. Band intensities were detected by the Western lightning plus-ECL detection system (PerkinElmer Inc, Waltham, MD, USA), recorded using the LuminoGraph II (Atto Corporation, Tokyo, Japan), and analyzed using the GeneTools of ChemiGenius (Syngene, Cambridge, UK).