Alkaline phosphatase conjugated goat anti mouse ige

Manufactured by Southern Biotech

Sourced in United States

Alkaline phosphatase-conjugated goat anti-mouse IgE is a laboratory reagent used for the detection and quantification of mouse immunoglobulin E (IgE) in various immunoassays. The product consists of goat-derived antibodies specific to mouse IgE that are conjugated with the enzyme alkaline phosphatase. This conjugation allows for the indirect detection of mouse IgE through the enzymatic activity of alkaline phosphatase, which can be measured using a suitable substrate.

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Lab products found in correlation

Ige antibodies, by Southern Biotech (2 mentions) P nitrophenyl phosphate substrate, by Merck Group (2 mentions) Rat anti mouse ige, by Southern Biotech (2 mentions) Spectrostar omega reader, by BMG Labtech (2 mentions) Natural der p 1, by Indoor Biotechnologies (1 mentions) Synergy h1 microplate reader, by Agilent Technologies (1 mentions) 4 nitrophenyl phosphate disodium salt hexahydrate, by Merck Group (1 mentions) Alkaline phosphatase conjugated goat anti mouse igm, by Southern Biotech (1 mentions) High binding eia ria plates, by Corning (1 mentions)

Spelling variants of this product

Goat anti-mouse IgE (11 citations) Alkaline phosphatase (6 citations) Anti-IgE (5 citations) Anti-mouse IgE (3 citations)

3 protocols using alkaline phosphatase conjugated goat anti mouse ige

Quantifying Anti-Der p 1 IgE Levels

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Anti-Der p 1-IgE was detected by coating plates with 2μg/mL natural Der p 1 (Indoor Biotechnologies), and serum antibody was detected using alkaline phosphatase-conjugated goat anti-mouse IgE (Southern Biotechnology Associates, AL). Total IgE levels were determined by coating plates with 2μg/mL rat anti-mouse IgE (Southern Biotechnology), and standard curves were prepared with known concentrations of IgE antibodies (Southern Biotechnology). For all ELISA assays, p-nitrophenyl phosphate substrate (Sigma) was added, and color development was detected with a SPECTROstar Omega Reader (BMG Labtech) at 405nm.

Patel P.S, & Kearney J.F. (2017). CD36 and Platelet Activating Factor Receptor (PAFR) Promote House Dust Mite Allergy Development. Journal of immunology (Baltimore, Md. : 1950), 199(3), 1184-1195.

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Quantifying HDM-specific IgE by ELISA

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Levels of circulating HDM allergen-specific IgE were assessed by ELISA. Briefly, 10 µg ml⁻¹ of HDM extract (Stallergenes Greer, NC, USA) was prepared in carbonate buffer (pH 9.6) and coated overnight at 4 °C in NUNC Immuno MaxiSorp plates. After a 2-h blocking step in PBS supplemented with 1% BSA, serum samples were diluted in PBS and incubated overnight at 4 °C. The next day, alkaline phosphatase-conjugated goat anti-mouse IgE (Southern Biotech, AL, USA) was diluted by 1’000 in PBS supplemented with 0.2% BSA and added to the samples for 2 h at room temperature. After addition of 4-Nitrophenyl phosphate disodium salt hexahydrate (Sigma- Aldrich), the colorimetric reaction was read at 405 nm on the Synergy H1 microplate reader (Biotek, Luzern, Switzerland).

Trompette A., Pernot J., Perdijk O., Alqahtani R.A., Domingo J.S., Camacho-Muñoz D., Wong N.C., Kendall A.C., Wiederkehr A., Nicod L.P., Nicolaou A., von Garnier C., Ubags N.D, & Marsland B.J. (2022). Gut-derived short-chain fatty acids modulate skin barrier integrity by promoting keratinocyte metabolism and differentiation. Mucosal Immunology, 15(5), 908-926.

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Quantifying Antibodies in Respiratory Samples

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ELISAs were performed to measure serum and BALF anti-α-1,3-glucan-IgM, and -IgA antibodies along with total IgE and anti-Bla g 2 IgE in the serum and total IgE in the BALF. Anti-α-1,3-glucan antibody ELISAs were conducted using high-binding EIA/RIA plates (Costar) coated with 2 μg/mL purified α-1,3-glucan. After incubation with serum or BALF, antibody binding was detected with alkaline phosphatase-conjugated goat anti-mouse IgM, (Southern Biotechnology, Birmingham, AL), or -IgA (Life Technologies). Standard curves were prepared using known quantities of α-1,3-glucan-specific IgM (A16) or IgA (J558) antibodies purified as described in Antibody reagents and flow cytometry. Anti-Bla g 2-IgE was detected by coating plates with 2 μg/mL natural Bla g 2 (Indoor Biotechnologies), and antibody was detected using alkaline phosphatase-conjugated goat anti-mouse IgE (Southern Biotechnology Associates, AL). Total IgE levels were determined by coating plates with 2 μg/mL rat anti-mouse IgE (Southern Biotechnology), and standard curves were prepared with known concentrations of IgE antibodies (Southern Biotechnology). For all ELISA assays, p-Nitrophenyl Phosphate Substrate (Sigma) was added, and color development was detected with a SPECTROstar Omega Reader (BMG Labtech) at 405nm.

Patel P.S., King R.G, & Kearney J.F. (2016). Pulmonary α-1,3-Glucan-Specific IgA-Secreting B Cells Suppress the Development of Cockroach Allergy. Journal of immunology (Baltimore, Md. : 1950), 197(8), 3175-3187.

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