In order to simulate the effect of the gastrointestinal digestion on the probiotic microorganisms, the methodology described in [17 ] was followed. Ti referred to a L. salivarius spp. salivarius content (CFU/g), meanwhile ti referred to a moment in the gastrointestinal digestion. Ten grams of the sample were mixed with 10 mL of pepsine 0.6% (w/v) (Sigma-Aldrich, Steinheim, Germany) adjusted to pH 3 with HCl 4M (t1—T1) and stirred at 37 °C for 90 min (t2—T2). The addition on a phosphate buffer solution (pH 8) with 10% of bile (Sigma-Aldrich, Steinheim, Germany) was performed in the third step (t3—T3). The phosphate buffer solution with 0.3% of bile and 0.1% pancreatine (Sigma-Aldrich, Steinheim, Germany) was added on following an incubation at 37 °C for 90 min (t4—T4). The results provided are the average of four replicates.
Phosphate buffer solution ph 8
Manufactured by Merck Group
Sourced in Germany
Phosphate buffer solution (pH 8) is a laboratory reagent used to maintain a specific pH environment in various experiments and applications. It is a balanced mixture of sodium phosphate and potassium phosphate salts that provides a stable pH of 8.0 at 25°C. This solution is commonly used in biochemical, cell culture, and analytical procedures where a controlled pH environment is required.
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Lab products found in correlation
2 protocols using phosphate buffer solution ph 8
1
Simulating Gastrointestinal Digestion of Probiotics
2
Gastrointestinal Digestion Effects on Microbial Survival
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The effect of the gastrointestinal digestion on the microorganism survival was determined following the procedure described in (Calabuig-Jiménez et al., 2019) . T i was the L. salivarius spp. salivarius content; t i was a moment during the gastrointestinal digestion. Briefly, 10 g of sample were mixed with 10 mL of pepsine (0.6% w/v) (Sigmaaldrich, Steinheim, Germany) adjusted to pH 3 with HCl 4 M (t 1 -T 1 ) and mixed at 37 °C for 90 min (t 2 -T 2 ). Phosphate buffer solution (pH 8) with 10% of bile (Sigma-aldrich, Steinheim, Germany) was added (t 3 -T 3 ). Phosphate buffer with 0.3% of bile and 0.1% pancreatine (Sigmaaldrich, Steinheim, Germany) was added following an incubation at 37 °C for 90 min (t 4 -T 4 ). The results provided are the average of four replicates.
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