The Washington University Genome Engineering and Induced Pluripotency Center (GEiC) derived multiple clonal iPSC lines from individuals in this family. Briefly, fresh urine samples were procured from the AP and UM and were used to obtain renal epithelial cells, which were reprogrammed using the CytoTune-iPSC 2.0 Sendai virus-based reprogramming kit (Thermo Fischer Scientific), as per the manufacturer’s instructions. iPSC clones were picked and three clonal cell lines were derived from each study subject. Clones number 1 and 3 from each subject were used for experimentation. These newly derived lines were compared with established male and female iPSC control lines from unrelated individuals (BJFF6 and AN1.1, denoted UC-M and UC-F in this study) provided by the Washington University Genome Engineering and Induced Pluripotency Center.
Cytotune ipsc 2.0 sendai virus based reprogramming kit
Manufactured by Thermo Fisher Scientific
The CytoTune-iPSC 2.0 Sendai virus-based reprogramming kit is a laboratory tool designed for the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). The kit utilizes a Sendai virus-based delivery system to introduce the necessary transcription factors for cell reprogramming.
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Lab products found in correlation
2 protocols using cytotune ipsc 2.0 sendai virus based reprogramming kit
1
Induced Pluripotent Stem Cell Lines
2
Derivation of iPSC Clones from Urine
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The Washington University Genome Engineering and Induced Pluripotency Center (GEiC) derived multiple clonal iPSC lines from individuals in this family. Briefly, fresh urine samples were procured from the AP and UM and were used to obtain renal epithelial cells, which were reprogrammed using the CytoTune-iPSC 2.0 Sendai virus-based reprogramming kit (Thermo Fischer Scientific), as per the manufacturer's instructions. iPSC clones were picked and three clonal cell lines were derived from each study subject. Clones number 1 and 3 from each subject were used for experimentation.
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