Hscrp elisa kit

Lipid and glucose levels were measured with a Siemens Dimension Chemistry Analyzer (Diamond Diagnostics, Holliston,MA, USA). Haemoglobin A1c (HbA1c) level were assessed with the Variant™ device (Bio-Rad, Hercules, CA, USA). hsCRP and Insulin levels were measured with the hsCRP ELISA kit (Biovendor, Asheville, NC, USA) and Mercodia Insulin ELISA (Mercodia AB,Uppsala, Sweden), respectively. Fetuin-A levels in plasma were assayed using ELISA (Biovendor, Modrice, Czech Republic). All assays were performed in accordance with the manufacturers’ instructions.

Glucose and lipid profiles were measured using a Siemens Dimension RXL chemistry analyzer (Diamond Diagnostics, Holliston, MA, USA). Hemoglobin A1c (HbA1c) levels were determined using the Variant™ device (BioRad, Hercules, CA, USA). Insulin and high-sensitivity CRP (hsCRP) levels were determined using a Mercodia Insulin ELISA Kit (Mercodia AB, Uppsala, Sweden) and an hsCRP ELISA kit (Biovendor, Asheville, NC, USA), respectively. Plasma levels of inflammatory and metabolic markers were measured using bead-based multiplexing technology on a Bioplex-200 system (BioRad). All of the aforementioned assays were performed according to the manufacturers’ instructions. The Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index was calculated using the following formula: HOMA-IR = (glucose × insulin)/22.5.

Circulating plasma levels of DUSP1 and oxLDL were determined using the human DUSP1 and oxLDL enzyme-linked immunosorbent assay (ELISA) kits (EIAab, Wuhan, China), and hsCRP levels were measured using a high-sensitivity CRP “hsCRP” ELISA kit (BioVendor, Asheville, NC). Assays were performed as per the manufacturers' instructions.