Human pyy total elisa kit

Manufactured by Merck Group

Sourced in United States

The Human PYY (Total) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of total PYY (Peptide YY) in human serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a microplate to capture the PYY analyte, which is then detected using a biotinylated detection antibody and a streptavidin-peroxidase conjugate. The assay provides a linear range and sensitivity suitable for PYY quantification.

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Lab products found in correlation

Human ghrelin total elisa cold packs, by Merck Group (2 mentions) Human insulin elisa kit, by Merck Group (1 mentions) Dpp4 inhibitor, by Merck Group (1 mentions) Multi species glp 1 total elisa kit, by Merck Group (1 mentions)

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Total PYY ELISAs

4 protocols using human pyy total elisa kit

Serum Hormone ELISA Analysis

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Serum hormone levels were determined using the sandwich ELISA technique performed by the following commercial kits according to the manufacturer’s instructions. Human Ghrelin (Total) ELISA COLD PACKS (Millipore, USA), Human PYY (Total) ELISA Kit (Millipore), and Human gastric inhibitory polypeptide (GIP) ELISA Kit (ENCO).

Oz-Levi D., Olender T., Bar-Joseph I., Zhu Y., Marek-Yagel D., Barozzi I., Osterwalder M., Alkelai A., Ruzzo E.K., Han Y., Vos E.S., Reznik-Wolf H., Hartman C., Shamir R., Weiss B., Shapiro R., Pode-Shakked B., Tatarskyy P., Milgrom R., Schvimer M., Barshack I., Imai D.M., Coleman-Derr D., Dickel D.E., Nord A.S., Afzal V., van Bueren K.L., Barnes R.M., Black B.L., Mayhew C.N., Kuhar M.F., Pitstick A., Tekman M., Stanescu H.C., Wells J.M., Kleta R., de Laat W., Goldstein D.B., Pras E., Visel A., Lancet D., Anikster Y, & Pennacchio L.A. (2019). Noncoding Deletions Expose a Novel Gene Critical for Intestinal Function. Nature, 571(7763), 107-111.

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Serum Hormone ELISA Analysis

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Measuring Gut Hormone Dynamics

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Total ghrelin was determined as previously described (Yanni et al., 2017 (link)). In brief, the protease inhibitor phenylmethanesulfonyl fluoride was added in K₃EDTA plasma immediately after isolation, and samples were then acidified with HCl and centrifuged for 10 min, at 3000 rpm and 4℃. A sandwich ELISA method (Human Ghrelin [Total] ELISA kit; Millipore) was used for the determination of total ghrelin at 0, 30, 60, 120, and 180 min. Total GLP‐1 and PYY were also detected in plasma and insulin in serum by sandwich ELISA methods using commercially available kits (Human Total Glucagon‐Like Peptide‐1 kit; Millipore, Human PYY [Total] ELISA kit; Millipore and Human Insulin ELISA kit; Millipore, respectively) at 0, 30, 60, 90, 120, and 180 min.
Glucose concentrations at 0, 15, 30, 45, 60, 90, 120, and 180 min were determined in plasma immediately by electrochemical method in an automated analyzer (ΥSI 2300 STAT PLUS).
At the beginning and the end of the dietary intervention, basal biochemical measurements were performed in serum by an automated biochemical analyzer (Yanni et al., 2019 (link)).

Yanni A.E., Konstantopoulos P., Kartsioti K., Binou P., Karathanos V.Τ., Chatzigeorgiou A, & Kokkinos A. (2021). Effects of 12‐week, non‐energy‐restricted dietary intervention with conventional yogurt οn appetite hormone responses of type 2 diabetic patients. Food Science & Nutrition, 9(12), 6610-6616.

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Appetite-associated hormone analysis protocol

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All blood samples were immediately transferred to disodium EDTA-treated tubes for analysis of appetiteassociated hormones. For the measure of PYY, GLP-1 and acylated ghrelin concentrations, test tubes were pretreated with the protease inhibitors DPP IV inhibitor (Millipore) and 4-(2-aminoethyl)benzenesulfonylfluoride hydrochloride (AEBSF, Alexis Biochemicals, Lausen, Switzerland). Blood was centrifuged at 906 g and at a temperature of 4°C for 15 min to isolate plasma. Plasma was separated and transferred to micro tubes for later analysis. Two micro tubes were pre-treated with hydrochloric acid (1 M, 100 µL per milliliter of plasma) to further protect acylated ghrelin from degradation. Plasma was stored at -70°C until hormone assays were conducted. Acylated ghrelin, total PYY and total GLP-1 were measured in duplicate using ELISA (Human Ghrelin(active) ELISA kit, Millipore; Human PYY(total) ELISA kit, Millipore; Multi Species GLP-1(total) ELISA kit, Millipore). The sensitivity of these ELISA kits were 8, 1.4 and 1.5 pg/mL, respectively, and the coefficient of variation values were 2.36, 5.26 and 3.28%, respectively.

Holliday A, & Blannin A. (2017). Appetite, food intake and gut hormone responses to intense aerobic exercise of different duration. The Journal of endocrinology, 235(3).

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