Copper chloride cucl 2 2h 2 o

Materials. Idarubicin hydrochloride, superoxide dismutase (SOD; 3,000 U/mg from bovine erythrocytes), catalase (45,000 U/mg from bovine liver) and cytochrome c (from equine heart) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Doxorubicin hydrochloride was obtained from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Plasmid DNA (pBR322) was obtained from Takara Bio Inc. (Kusatsu, Japan) and DNA gel loading dye (6x) was from Toyobo Co., Ltd. (Osaka, Japan). Calf thymus DNA was obtained from Worthington Biochemical Co. (Lakewood, NJ, USA) and copper chloride (CuCl 2 •2H 2 O) was from Nacalai Tesque Inc. (Kyoto, Japan). Diethylenetriamine-N,N,N',N'',N''-penta-acetic acid (DTPA) and bathocuproinedisulfonic acid were obtained from Dojindo Laboratories Co. (Kumamoto, Japan) and methional [3-(methylthio) propionaldehyde] was from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All other chemicals used were of the highest grade commercially available.

Materials. Pirarubicin, superoxide dismutase (SOD; 3,000 U/mg from bovine erythrocytes), catalase (45,000 U/mg from bovine liver) and cytochrome c (from equine heart) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Plasmid DNA (pBR322) and DNA gel loading dye (6×) were from Toyobo Co. (Osaka, Japan). Copper chloride (CuCl 2 •2H 2 O) was from Nacalai Tesque Co (Kyoto, Japan). Diethylenetriamine-N,N,N',N'',N''-penta-acetic acid (DTPA) and bathocuproinedisulfonic acid were from Dojin Chemicals Co. (Kumamoto, Japan). 3-(Methylthio) propionaldehyde (methional) was from Tokyo Kasei Co. (Tokyo, Japan). All other chemicals used were of the highest purity commercially available.