Trimethylchlorosilane

All reagents and solvents were super purity or HPLC grade unless otherwise stated. Anhydrous pyridine, 2',7'-dichlorofluorescein solution, dichloromethane, and hexamethyl disilazane were from Honeywell (Fisher Scientific SL, Madrid, Spain). Acetone, ethanol, ethyl acetate, ethyl ether, methanol, n-hexane, n-heptane, propan-2-ol, propionitrile and trimethylchlorosilane were supplied by VWR International, LLC (West Chester, Pennsylvania, USA), anhydrous sodium sulfate and potassium hydroxide by Panreac Quimica, S.A.U. (Castellar del Valles, Barcelona, Spain). The standards 5α-cholestan-3ß-ol, α-, ß-, γ-, and δ-tocopherol, and n-eicosane (C20) were purchased at Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Silica gel 60 for column chromatography, 70-230 mesh (Merck KGaA, Darmstadt, Germany) was used directly from the container. Glass chromatography columns (50 cm length x 1.5 cm i.d.) were supplied with Teflon stopcocks; a plug of glass wool fiber was placed at the bottom and everything was washed with n-hexane before use.

All reagents and solvents were of recognized analytical quality and the water used was ultrapure. Anhydrous pyridine (Py), chloroform (CHL), dichloromethane (DM), diethyl ether (DEE), hexamethyldisilazane (HMDS), hexane (Hex), methanol (MeOH), and trimethylchlorosilane (TMC) were purchased from VWR International, LLC (West Chester, Pennsylvania, USA). Phenolphthalein, potassium hydroxide (titrated 0.1 mol/L KOH ethanolic solution), the internal standard (IS) 1,3dipalmitoyl-glycerol (1,3-PP), and the solid phase extraction (SPE) diol cartridges (3 mL) were obtained at Sigma-Aldrich (Merck KGaA, Darmastadt, Germany).