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Cd160 7h1

Manufactured by Miltenyi Biotec

CD160 (7H1) is a monoclonal antibody produced by Miltenyi Biotec. It is designed for the detection and analysis of CD160 expression on cells.

Automatically generated - may contain errors

2 protocols using cd160 7h1

1

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were procured from BioLegend: CD44 (IM7), CD62L (MEL-14), CD127 (A7R34), T-bet (4B10), PD-1 (RMP1–30), CD160 (7H1), TIM3 (RMT3–23), CD3ε (17A2), TNFα (MP6-XT22), CD8α (53–6.7), CD4 (RM4–5), CD45.1 (A29), CD45.2 (104); Miltenyi Biotec: TOX (REA473); Southern Biotech: KLRG1 (2F1); eBioscience: Eomes (Dan11mag), 2B4 (eBio244F4), IFNγ (XMG1.2), Granzyme B (GB11), B220 (RA3–6B2); or from BD Biosciences: TIGIT (1G9), LAG33 (C9B7W), TCF1 (S33–966), 2B4 (2B4), Ki-67 (B56). Live cells were discriminated by staining with Zombie NIR dye (BioLegend). Intracellular and nuclear staining of cytokines, effector molecules, and transcription factors was performed using the FoxP3/Transcription Factor Staining Buffer Set (eBioscience) in accordance with the manufacturer’s protocol. Flow cytometry data were acquired on a BD LSR II instrument and cell sorting was performed on a BD FACSAria enclosed within a laminar flow hood. Data were analyzed using FlowJo software (TreeStar).
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2

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were procured from BioLegend: CD44 (IM7), CD62L (MEL-14), CD127 (A7R34), T-bet (4B10), PD-1 (RMP1–30), CD160 (7H1), TIM3 (RMT3–23), CD3ε (17A2), TNFα (MP6-XT22), CD8α (53–6.7), CD4 (RM4–5), CD45.1 (A29), CD45.2 (104); Miltenyi Biotec: TOX (REA473); Southern Biotech: KLRG1 (2F1); eBioscience: Eomes (Dan11mag), 2B4 (eBio244F4), IFNγ (XMG1.2), Granzyme B (GB11), B220 (RA3–6B2); or from BD Biosciences: TIGIT (1G9), LAG33 (C9B7W), TCF1 (S33–966), 2B4 (2B4), Ki-67 (B56). Live cells were discriminated by staining with Zombie NIR dye (BioLegend). Intracellular and nuclear staining of cytokines, effector molecules, and transcription factors was performed using the FoxP3/Transcription Factor Staining Buffer Set (eBioscience) in accordance with the manufacturer’s protocol. Flow cytometry data were acquired on a BD LSR II instrument and cell sorting was performed on a BD FACSAria enclosed within a laminar flow hood. Data were analyzed using FlowJo software (TreeStar).
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