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Mouse monoclonal anti ryr antibody 34c

Manufactured by Thermo Fisher Scientific
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The Mouse monoclonal anti-RyR antibody 34C is a laboratory reagent used to detect the presence of ryanodine receptor (RyR) proteins in biological samples. It is a mouse-derived monoclonal antibody that specifically binds to RyR. The antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, or immunocytochemistry, to identify and localize RyR proteins in cells or tissues.

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Lab products found in correlation

Hrp conjugated anti flag m2 antibody, by Merck Group (2 mentions) Mouse monoclonal anti actin antibody, by Merck Group (1 mentions) Anti flag m2 antibody, by Merck Group (1 mentions) Anti bax, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-RyR Monoclonal mouse antibody

2 protocols using mouse monoclonal anti ryr antibody 34c

1

Bcl-XL Binding Peptide Evaluation

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Unless otherwise specified, all chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). The following antibodies were used: mouse monoclonal anti-actin antibody, anti-FLAG M2 antibody and HRP-conjugated anti-FLAG M2 antibody (Sigma-Aldrich), mouse monoclonal anti-RyR antibody 34C (Thermo Scientific, Rockford, IL, USA, or Developmental Studies Hybridoma Bank, University of Iowa, Iowa, USA) and mouse monoclonal anti-Bcl-XL antibody YTH-2H12 (Trevigen, Gaithersburg, WV, USA). The sequences of the peptides used in this study were:
Biotin-BH4-Bcl-XL: Biotin-MSQSNRELVVDFLSYKLSQKGYSW (also used without the biotin tag)
Biotin-scrambled BH4-Bcl-XL: Biotin-WYSKQRSLSGLVMYVLEDKNSQFS
Control peptide: WYEKQRSLHGIMYYVIEDRNTKGYR
These peptides were synthesized by Life Tein (Hillsborough, NJ, USA) with a purity of at least 85%.
Vervliet T., Lemmens I., Vandermarliere E., Decrock E., Ivanova H., Monaco G., Sorrentino V., Kasri N.N., Missiaen L., Martens L., De Smedt H., Leybaert L., Parys J.B., Tavernier J, & Bultynck G. (2015). Ryanodine receptors are targeted by anti-apoptotic Bcl-XL involving its BH4 domain and Lys87 from its BH3 domain. Scientific Reports, 5, 9641.
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2

Immunoprecipitation and Antibody Detection

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Unless otherwise mentioned all chemicals were obtained from Sigma-Aldrich. The antibodies used in this study include: HRP conjugated anti-FLAG M2 antibody (Sigma-Aldrich), mouse monoclonal anti-RyR antibody 34C (Thermo Scientific, Rockford, IL, USA, or Developmental Studies Hybridoma Bank, University of Iowa, USA) and rabbit polyclonal anti-Bax (Santa Cruz). The peptide used for elution of the FLAG pull-downs was MDYKDHDGDYKDHDIDYKDDDDK (Life Tein).
Vervliet T., Lemmens I., Welkenhuyzen K., Tavernier J., Parys J.B, & Bultynck G. (2015). Regulation of the ryanodine receptor by anti-apoptotic Bcl-2 is independent of its BH3-domain-binding properties. Biochemical and biophysical research communications, 463(3).
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