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Mtch2

Manufactured by Proteintech
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MTCH2 is a laboratory consumable product offered by Proteintech. It functions as a molecular target of cholesterol homeostasis protein 2. The core purpose of this product is to facilitate research and experimentation related to the MTCH2 protein and its biological role.

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Lab products found in correlation

Tom40, by Abcam (3 mentions) Pdhe1 a, by Abcam (3 mentions) Tom20, by Santa Cruz Biotechnology (3 mentions) Beta actin, by Boster Bio (3 mentions) Mmp 9, by Cell Signaling Technology (3 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (3 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (3 mentions) Total oxphos rodent antibody cocktail, by Abcam (2 mentions) Phospho s6 ser240 244, by Cell Signaling Technology (2 mentions) 4 hne, by Abcam (2 mentions) Bcl2 associated x (bax), by Merck Group (2 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (2 mentions) Phospho akt thr308, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) N cadherin, by Cell Signaling Technology (2 mentions) Vimentin, by Cell Signaling Technology (2 mentions) Phospho akt ser473, by Cell Signaling Technology (2 mentions) Bcl 2, by Santa Cruz Biotechnology (2 mentions) Cofilin 1, by Cell Signaling Technology (1 mentions) Total oxphos cocktail, by Abcam (1 mentions)

4 protocols using mtch2

1

Quantifying Protein Expression Profiles

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Total cells were lysed with 2% SDS solution plus protease and phosphatase inhibitors (Thermo Scientific). Protein concentrations were tested by BCA kit and equivalent proteins were loaded into SDS-PAGE. Following western blots were performed according to standard procedures. The primary antibodies were list as follow: MTCH2 (Proteintech, Cat#16888-1-AP), Beta actin (Boster Biological Technology, Cat#BM0627), PDHE1-A (Abcam, Cat#ab110334), GFAP (Millipore, Cat#G3893), Tom20 (Santa Cruz Biotechnology, Cat#sc-136211), Tom40 (Abcam, Cat#ab185543), MMP-9 (Cell Signaling Technology, Cat#3852), N-cadherin (Cell Signaling Technology, Cat#4061), Vimentin (Cell Signaling Technology, Cat#5741), 4-HNE (Abcam, Cat#ab48506), BcL-2 (Santa Cruz Biotechnology, Cat#sc-7382), Bax (Millipore, Cat#06-499), phospho-AKT (Thr308) (Cell Signaling Technology, Cat#2965), phospho-AKT (Ser473) (Cell Signaling Technology, Cat#4060), AKT (Cell Signaling Technology, Cat#9272), phospho-S6 (Ser240/244) (Cell Signaling Technology, Cat#2215), S6 (Cell Signaling Technology, Cat#2217), phospho-4EBP1 (Thr37/46) (Cell Signaling Technology, Cat#9459), total OXPHOs rodent antibody cocktail (Abcam, Cat#110413), phospho-Cofilin1 (Ser3) (Cell Signaling Technology, Cat#3313), Cofilin1 (Cell Signaling Technology, Cat#5175), PARP (Abclonal, Cat#A0942) and cleaved caspase 3 (Cell Signaling Technology, Cat#9661).
Yuan Q., Yang W., Zhang S., Li T., Zuo M., Zhou X., Li J., Li M., Xia X., Chen M, & Liu Y. (2021). Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma. Molecular Medicine, 27, 7.
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2

Multiparametric Mitochondrial Profiling

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BNIP3 (Abcam, ab109362; Abcam, ab10433; Cell signaling, 3769S), NIX (Cell signaling, 12396S), FLAG (Sigma-Aldrich, F1804), HA (Sigma-Aldrich, H6533), ACTIN (Sigma-Aldrich, A2066), TOM70 (Proteintech, 14528-1-AP), TOM20 (ABclonal, A19403), SMAC (Cell signaling, 2954S), MITOFILIN (Proteintech, 10179-1-AP), HSP60 (Cell signaling, 4870S), TIMM23 (Proteintech, 11123-1-AP), SKP1 (Cell signaling, 2156S), CUL1 (Proteintech, 12895-1-AP), Total OXPHOS Cocktail (Abcam, ab110411), Cytochrome C (BD Biosciences, 556433), mCherry (Easybio, BE2026), MTCH2 (Proteintech, 16888-1-AP), BECLIN1 (Cell signaling, 3495T), FIP200 (ABclonal, A14685), TFAM (Abcam, ab131607), LONP1 (Proteintech, 15440-1-AP), VDAC (Cell signaling, 4866S), PARP (Cell signaling, 9542S), Donkey polyclonal anti-Rabbit IgG (H+L), , Donkey polyclonal anti-Mouse IgG (H+L), HRP-conjugated (Jackson ImmunoResearch, 711-035-151), Goat polyclonal anti-Mouse IgG, Fcγ fragment specific, HRP-conjugated (Jackson ImmunoResearch, 115-035-008), Goat polyclonal anti-Mouse IgG, light chain specific, HRPconjugated (Jackson ImmunoResearch, .
Cao Y., Zheng J., Wan H., Sun Y., Fu S., Liu S., He B., Cai G., Cao Y., Huang H., Li Q., Ma Y., Chen S., Wang F, & Jiang H. (2023). A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease. The EMBO journal, 42(13).
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3

Protein Expression Profiling Protocol

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Total cells were lysed with 2% SDS solution plus protease and phosphatase inhibitors (Thermo Scienti c). Protein concentrations were tested by BCA kit and equivalent proteins were loaded into SDS-PAGE. Following western blots were performed according to standard procedures. The primary antibodies were list as follow: MTCH2 (Proteintech, Cat#16888-1-AP), Beta actin (Boster Biological Technology, Cat#BM0627), PDHE1-A (Abcam, Cat#ab110334), GFAP (Millipore, Cat#G3893), Tom20 (Santa Cruz Biotechnology, Cat#sc-136211), Tom40 (Abcam, Cat#ab185543), MMP-9 (Cell Signaling Technology,
Yuan Q., Yang W., Zhang S., Li T., Zuo M., Zhou X., Li J., Mao L., Xia X., Chen M., & Liu Y. (2020). Inhibition of Mitochondrial Carrier Homolog 2 (MTCH2) Suppresses Tumor Invasion and Enhances Sensitivity to Temozolomide in Malignant Glioma.
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4

Comprehensive Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cells were lysed with 2% SDS solution plus protease and phosphatase inhibitors (Thermo Scienti c). Protein concentrations were tested by BCA kit and equivalent proteins were loaded into SDS-PAGE. Following western blots were performed according to standard procedures. The primary antibodies were list as follow: MTCH2 (Proteintech, Cat#16888-1-AP), Beta actin (Boster Biological Technology, Cat#BM0627), PDHE1-A (Abcam, Cat#ab110334), GFAP (Millipore, Cat#G3893), Tom20 (Santa Cruz Biotechnology, Cat#sc-136211), Tom40 (Abcam, Cat#ab185543), MMP-9 (Cell Signaling Technology, Cat#3852), N-cadherin (Cell Signaling Technology, Cat#4061), Vimentin (Cell Signaling Technology, Cat#5741), 4-HNE (Abcam, Cat#ab48506), BcL-2 (Santa Cruz Biotechnology, Cat#sc-7382), Bax (Millipore, Cat#06-499), phospho-AKT (Thr308) (Cell Signaling Technology, Cat#2965), phospho-AKT (Ser473) (Cell Signaling Technology, Cat#4060), AKT (Cell Signaling Technology, Cat#9272), phospho-S6 (Ser240/244) (Cell Signaling Technology, Cat#2215), S6 (Cell Signaling Technology, Cat#2217), phospho-4EBP1 (Thr37/46) (Cell Signaling Technology, Cat#9459), total OXPHOs rodent antibody cocktail (Abcam, Cat#110413), phospho-Co lin1 (Ser3) (Cell Signaling Technology, Cat#3313), Co lin1 (Cell Signaling Technology, Cat#5175), PARP (Abclonal, Cat#A0942) and cleaved caspase 3 (Cell Signaling Technology, Cat#9661).
Yuan Q., Yang W., Zhang S., Li T., Zuo M., Zhou X., Li J., Mao L., Xia X., Chen M., & Liu Y. (2020). Inhibition of Mitochondrial Carrier Homolog 2 (MTCH2) Suppresses Tumor Invasion and Enhances Sensitivity to Temozolomide in Malignant Glioma .
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