Neutrophil samples were stained with anti-MPO-FITC antibody and mounted in DAPI-containing ProLong Gold Antifade Mountant (Thermo Fisher Scientific). AIM2 and IFI16 proteins were expressed, purified, and fluorescently labeled as previously described (Morrone et al., 2014 (link); Morrone et al., 2015 (link)). SLE renal biopsies were stained as previously described (Antiochos et al., 2018 (link)) using anti-MPO rabbit polyclonal (ThermoFisher), anti-MPO mouse monoclonal (ThermoFisher), anti-IFI16 mouse monoclonal (Sigma), anti-AIM2 rabbit polyclonal (Sigma), and Hoechst 33,342 (ThermoFisher). Confocal imaging was performed with a Zeiss AxioObserver with 780-Quasar confocal module.
780 quasar confocal module
Manufactured by Zeiss
The 780-Quasar confocal module is a high-performance microscopy tool designed for advanced imaging applications. It provides a versatile and efficient platform for confocal fluorescence imaging, enabling researchers to capture detailed, high-resolution images of their samples.
Automatically generated - may contain errors
Lab products found in correlation
Axio observer, by Zeiss (3 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions)
C apochromat 40 na 1.2 water objective, by Zeiss (1 mentions)
Axiovert 200, by Zeiss (1 mentions)
Lsm 700 confocal module, by Zeiss (1 mentions)
3 protocols using 780 quasar confocal module
1
Neutrophil and Renal Biopsy Staining
2
Fluorescence Correlation Spectroscopy of mCherry-myoII in Dictyostelium
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Dictyostelium cells transformed with mCherry-myoII were plated on glass dishes. FCS experiments were performed on interphase cells at ambient temperature (∼20–23°C; normal growth temperatures for Dictyostelium cells) using a Zeiss AxioObserver with 780-Quasar confocal module and FCS, with a C-Apochromat 40× (NA 1.2) water objective. 100 nM Rhodamine 6G was used for pinhole alignment and structural parameter calculation. A structural parameter of six was used, and the confocal volume was measured to be 0.45 fL. Data were fit to a single-component 3D diffusion model, including a triplet-state component with an upper limit of 8 µs (Srivastava and Robinson, 2015 (link); Kothari et al., 2017 (link)).
3
Multi-Modal Confocal Microscopy Protocols
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Confocal microscopy was performed on a Zeiss AxioObserver with 780-Quasar confocal module & FCS (NIH Grant S10 OD016374), a Zeiss AxioObserver with LSM700 confocal module (NIH Grant S10 OD016374), or a Zeiss Axiovert 200 with 510-Meta confocal module. All DIC microscopy was performed with a Zeiss Axiophot 2 with Janoptik ProgResC14 Plus opitical imaging system.
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