We purchased BSA from Sigma-Aldrich; KMnO4 from Sinopharm Chemical Reagent CO., Ltd. (Shanghai, China); DOX from Shanghai Aladdin Bio-Chem Technology CO., Ltd. (Shanghai, China); Daunorubicin (DNR) from Dalian Meilun Biotech Co., Ltd. (Dalian, China); Indocyanine green (ICG) from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan); Fetal bovine serum (FBS) from Elite (Marburg, Germany); Roswell Park Memorial Institute 1,640 (RPMI 1640) as well as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) from Keygen Biotech Corp., Ltd. (Jiangsu, China). The Milli-Q system (Millipore, United States) purified the water. Other analytical grade chemicals were obtained from Sigma (St. Louis, United States) and utilized with no additional purification except as described elsewhere.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
Manufactured by Keygen Biotech
Sourced in China
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a water-soluble tetrazolium salt. It is used as a colorimetric assay for assessing cell metabolic activity.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Milli q system, by Merck Group (2 mentions)
Rpmi 1640, by Keygen Biotech (2 mentions)
Indocyanine green icg, by Tokyo Chemical Industry (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Kmno4, by Sinopharm (1 mentions)
Dimethylaminopyridine dmap, by Sinopharm (1 mentions)
Curcumin, by Adamas (1 mentions)
Rpmi 1640 medium, by Keygen Biotech (1 mentions)
Hoechst 33258, by Beyotime (1 mentions)
Paraformaldehyde (pfa), by Beyotime (1 mentions)
Lyso tracker red, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Keygen Biotech (1 mentions)
Pluronic f68, by BASF (1 mentions)
Tween 80, by Merck Group (1 mentions)
Tetraethyl orthosilicate teos, by Merck Group (1 mentions)
K562 adr cells, by Keygen Biotech (1 mentions)
Annexin 5 fitc pi, by Keygen Biotech (1 mentions)
Xylose, by Merck Group (1 mentions)
Monosaccharide standard, by Merck Group (1 mentions)
11 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
1
Nanomedicine Synthesis and Evaluation
2
Synthesis and Characterization of CTPP-Curcumin Nanoparticles
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3-Carboxypropyl-triphenylphosphonium bromide (CTPP) was obtained from Adamas Reagent Co., Ltd (Shanghai, China). Polyethylene glycol (PEG), ε-caprolactone was obtained from Daigang Biomaterial (Shandong, China). N,N′-Dicyclohexylcarbodimide (DCC) and dimethylaminopyridine (DMAP) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Curcumin was purchased from Adamas Reagent Co., Ltd (Shanghai, China). LysoTracker Red, 4% paraformaldehyde and Hoechst 33258 were obtained from Beyotime Biotechnology (Shanghai, China). RPMI-1640 medium and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from KeyGen Biotech (Nanjing, China). Fetal bovine serum (FBS) was purchased from Gibco (Hangzhou, China). All the other chemicals and reagents were analytic grade.
3
Cytotoxicity Evaluation of Anticancer Drug
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Pluronic F68 (molecular weight 8,400) was a gift from BASF Co, Ltd, (Mannheim, Germany). Cholesterol chloroformate was purchased from Tokyo Chemical Industry Co, Ltd, (Tokyo, Japan). Cabazitaxel was sourced from Taizhou Baolong Chemical Co, Ltd, (Zhejiang, People’s Republic of China). Tween 80 was obtained from Merck KGaA Co, Ltd, (Darmstadt, Germany). 4-Dimethylaminopyridine, triethylamine, and dichloromethane were purchased from Shandong Yuwang Co, Ltd, (Shandong, People’s Republic of China). 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), Roswell Park Memorial Institute 1640 medium (RPMI 1640), and fetal bovine serum were purchased from KeyGen Biotech Co, Ltd, (Nanjing, People’s Republic of China). S180 cells were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, People’s Republic of China). All other reagents were of analytical grade and obtained from commercial sources.
Male Kunming mice weighing 18–22 g were purchased from the Central Animal Laboratory of Shenyang Pharmaceutical University (Shenyang, People’s Republic of China). All mice had free access to food and water. All experiments were carried out in accordance with the guidelines of the local animal welfare committee.
Male Kunming mice weighing 18–22 g were purchased from the Central Animal Laboratory of Shenyang Pharmaceutical University (Shenyang, People’s Republic of China). All mice had free access to food and water. All experiments were carried out in accordance with the guidelines of the local animal welfare committee.
4
Doxorubicin-Loaded Silica Nanoparticles
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CaCl2 (AR), Na2HPO4·12H2O (AR) NaOH (AR) ethanol (AR) were purchased from Nanjing Chemistry Reagent co., Ltd. Tetraethyl orthosilicate (TEOS) and aminopropyltriethoxysilane (APTES) were purchased from Aldrich. Doxorubicin (DOX), 1640 medium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Annexin V-FITC/PI were purchased from KeyGen Biotech Co. (Nanjing, China). All aqueous solutions were prepared using DEPC treated ultrapure water from a Milli-Q system (Millipore, USA). The siRNA duplex consists of 5′-r(CGGAAGGCCUAAUGCCGAA)dTdT (sense) 5′-r(UUCGGCAUUAGGCCUUCCG)dTdT (antisense) was obtained from Shanghai GenePharma (Shanghai, China). K562 and K562/ADR cells were supplied by KeyGen Biotech Co. Ltd. (Nanjing, China). All the biological regents used in RT-PCR and WB were obtained from KeyGen Biotech Co. Ltd. (Nanjing, China).
5
Wolfberry Pollen Polysaccharide Extraction
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The wolfberry pollen was collected from the Ningxia Academy of Agriculture and Forestry Sciences (Yinchuan, Ningxia, China). Cellulose DEAE-52, Sephadex G-100 columns and monosaccharide standards (mannose, glucuronic acid, galacturonic acid, xylose, galactose, arabinose, and trehalose) were purchased from Sigma Chemical Co., Ltd. (St. Louis, MO, USA). HPLC-grade acetonitrile was purchased from TEDIA Co., Inc. (Fairfield, IA, USA). 1-phenyl-3-methyl-5-pyrazolone (PMP) high-glucose DMEM medium and fetal bovine serum were purchased from Gibco (Carlsbad, CA, USA). Penicillin-streptomycin double antibody and 0.25% trypsin were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from KeyGEN BioTech Co., Ltd. Nanjing, China). In situ cell death detection kit and POD were purchased from Roche Diagnostics (Indianapolis, IN, USA) and DAPI was obtained from ZSGB-BIO Biological Co., Ltd. (Beijing, China). Annexin V-FITC cell apoptosis detection kit was purchased from Best Bio Biological Co., Ltd. (Shanghai, China).
6
Multifunctional Nanoparticle Drug Delivery
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Quercetin (Que), imidazole and Traut's Reagent were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Triphenylphosphine (TPP) was purchased from Alfa Aesar Co., Inc. (Ward Hill, MA, USA). Sodium iodide, cesium carbonate and 1-bromo-4-chlorobutane were all purchased from J&K Co., Ltd. (Beijing, China). Alpha-maleinimido-omega-carboxy succinimidyl ester poly (ethylene glycol) (NHS–PEG–Mal, Mw = 2000 Da) was obtained from Shanghai yarebio Co., Ltd. (Shanghai, China). 4-Aminophenylboronic acid was obtained from Shanghai aladdin Co., Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX) was purchased from China Langchem Inc. (Shanghai, China). Anti-VEGF mAb was kindly provided by Prof. Yu Liu.43 RPMI 1640 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were both purchased from KeyGEN Biotech (Nanjing, China). Fetal bovine serum (FBS) was purchased from Gibco (Burlington, ON, Canada). All other chemicals and reagents in this study were analytical grade.
7
Bioconjugation of Quantum Dots
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Hydrophobic quantum dots (QDs) were purchased from Suzhou Xingshuo Nanotech Co., Ltd. Green fluorescence-emitting QDs had a fluorescence peak wavelength at 520 nm, and red fluorescence-emitting QDs had a fluorescence peak wavelength at 620 nm. The surface ligand was trioctylphosphine oxide (TOPO) for both green and red QDs. Bovine serum albumin (BSA), bovine α-lactalbumin (BLA), benzyl ether and oleic acid were purchased from Sigma-Aldrich. Iron(iii ) acetylacetonate was purchased from Tokyo Chemical Industry Co., Ltd. 1,2-Hexadecanediol was purchased from J&K Chemical Ltd. (Shanghai). Oleylamine was purchased from Kuer Bio-Engineering Co., Ltd. N-Hydroxysulfosuccinimide (NHS) sodium salt, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC), 2-(N-morpholino)ethanesulfonic acid (MES) buffer and doxorubicin hydrochloride (DOX) were purchased from Aladdin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from KeyGENBioTECH. Tetrahydrofuran (THF), hexane and absolute ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd. The RGD peptide (sequence Arg–Gly–Asp) was purchased from ChinaPeptides. Cell lines (HeLa and U87MG) and their culture media were purchased from KeyGENBioTECH.
8
Breast Cancer Cell Apoptosis Assay
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Bi(NO3)3·5H2O and NaBH4 were purchased from Aladdin Biochemical Technology Co., Ltd. 1,2‐distearoyl‐sn‐glycero‐3‐phosphorylethanolamine (DSPE) and phosphatidylcholine (PC) were purchased from A.V.T Pharmaceutical Co., Ltd. Other chemicals were purchased from Xilong Scientific Co., Ltd.
4T1 breast cancer cells were purchased from KeyGEN Bio TECH Co., Ltd. and cultured in RPMI 1640 complete media supplemented with 10% foetal bovine serum, 2 mM of L‐Glutamine, and 1% of the penicillin‐streptomycin solution. Cells were maintained at 37°C in a humidified atmosphere with 5% CO2. RIPA Lysis Buffer and BCA protein assay kit were purchased from Beyotime Biotechnology Co., Ltd. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) was purchased from Jiangsu KeyGEN BioTECH Co., Ltd. Anti‐Cleaved Caspase 3 antibody [E83‐77], anti‐HSP‐70 antibody [5A5], and anti‐p‐p65 antibody [EP2294Y] were purchased from Abcam.
4T1 breast cancer cells were purchased from KeyGEN Bio TECH Co., Ltd. and cultured in RPMI 1640 complete media supplemented with 10% foetal bovine serum, 2 mM of L‐Glutamine, and 1% of the penicillin‐streptomycin solution. Cells were maintained at 37°C in a humidified atmosphere with 5% CO2. RIPA Lysis Buffer and BCA protein assay kit were purchased from Beyotime Biotechnology Co., Ltd. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) was purchased from Jiangsu KeyGEN BioTECH Co., Ltd. Anti‐Cleaved Caspase 3 antibody [E83‐77], anti‐HSP‐70 antibody [5A5], and anti‐p‐p65 antibody [EP2294Y] were purchased from Abcam.
9
Curcumin-Loaded Nanomedicine for Cancer Therapy
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Methoxy poly(ethylene glycol) amine (mPEG-NH2, Mw 5 kDa) was obtained from Xiamen Sinopeg Biotech Co., Ltd. (Xiamen, China). HPBA and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) were purchased from Energy Chemical Co., Ltd. (Shanghai, China). N-Hydroxysuccinimide (NHS) and Curcumin (Cur) were purchased from Aladdin Industries Inc. (Shanghai, China). Poly(acrylic acid) (PAA, Mw 5 kDa) and 4-dimethylaminopyridine (DMAP) were obtained from J&K Scientific Ltd. (Beijing, China). RPMI 1640, 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide (MTT), and Hoechst 33342 were purchased from KeyGENBioTECH (Nanjing, China). LysoTracker® Red DND-99 and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). N-acetyl-cysteine (NAC) was purchased from Tianjin Heowns Biochemical Technology Co., Ltd. (Tianjin, China). All other chemicals and reagents were obtained from commercial sources with the highest purity available. A549 cells were purchased from cell storeroom of Chinese Academy of Sciences (Shanghai, China).
10
Photodynamic Therapy for Breast Cancer
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Dopamine hydrochloride, ammonia aqueous solution (NH4OH, 28–30% wt%), Chlorin e6 (Ce6) and cell uptake inhibitors (nocodazole, cytochalasin D, chlorpromazine, genistein, and β-cyclodextran) were purchased from Sigma-Aldrich (Shanghai, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was provided by KeyGEN Biotech. Co., Ltd. (Jiangsu, China). Amine-PEG-thiol (NH2-PEG-SH, MW ≈ 2000 Da) was obtained from Laysan Bio. Inc. (Shanghai, China). Singlet oxygen sensor green (SOSG) kits were purchased from Invitrogen (Shanghai, China). Human breast tumor cells (SKBR-3) and human mammary epithelial cells (MCF 10A) were purchased from ATCC (Manassas, VA). Fetal bovine serum (FBS), trypsin, media and phosphate buffered saline (PBS) were purchased from Gibco/Life Technologies (Shanghai, China).
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