The A549 cell line were obtained from ECACC and maintained in DMEM or EMEM, respectively, supplemented with 2 mM of glutamine, 10% fetal bovine serum, and antibiotics (1% of 100 U/L penicillin, 100 mg/mL streptomycin). L929 (NCTC clone 929, ATCC® CCL-1™) and HeLa (ATCC® CCL-2™) cell lines were obtained from ATCC and maintained in EMEM supplemented with antibiotics (1% of 100 U/L penicillin, 100 mg/mL streptomycin) and with 5% and 10% FBS respectively. Cell line was routinely grown in tissue culture flasks (75 cm2) and kept in a humidified atmosphere of 5% CO2 at 37 °C. All examined cell lines was tested against mycoplasma contamination with microbiological assays. The research was carried out using the normal cell line L929—mouse fibroblast and cancer cells: HeLa—human cervical cancer, A549—human lung cancer. All of the stock solutions of the tested compounds were dissolved in DMSO.
Atcc ccl 1
Manufactured by American Type Culture Collection
Sourced in United States
ATCC® CCL-1™ is a cell line derived from the HeLa cell line, which was originally isolated from a human cervical carcinoma. The cell line is commonly used for various cell biology and biomedical research applications.
Automatically generated - may contain errors
Lab products found in correlation
Atcc crl 1573, by American Type Culture Collection (2 mentions)
L glutamine, by Merck Group (2 mentions)
Atcc ccl 2, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Minimum essential medium eagle, by Merck Group (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Atcc tib 71, by American Type Culture Collection (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Williams e medium, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Insulin, by Thermo Fisher Scientific (1 mentions)
Selenium, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Sartorius (1 mentions)
Aria flow cytometer, by BD (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Epigallocatechin 3 gallate, by Merck Group (1 mentions)
Cell culture flasks and plates, by Corning (1 mentions)
16 protocols using atcc ccl 1
1
Cell Culture Protocols for Cancer Research
2
Culturing Murine Fibroblast L-929 Cells
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A murine fibroblast L-929 cell line was purchased from ATCC (ATCC® CCL1 ™; 10801 University Boulevard Manassas VA, 20110, USA). Cells were grown in culture flasks containing Minimum Essential Medium Eagle (Millipore Sigma, Burlington, MA, USA), supplemented with 10% fetal bovine serum (FBS, Millipore Sigma, Burlington, MA, USA) and 1% antibiotic-antimytotic solution (Millipore Sigma, Burlington, MA, USA). Cells were maintained at +37 °C in a humidified 5% CO2 atmosphere and monitored daily by using an inverted microscope. Subcultures were performed when 80% of confluence was observed.
3
Cell Line Cultivation Protocol
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Fibroblast (L929) cell line (ATCC®-CCl1), macrophages (RAW 264.7) (ATCC® TIB-71), and human embryonic renal cells (HEK-293) transfected with a luciferase-expressing gene (ATCC® CRL-1573) were obtained from Cell Line Service, Brazil. The cells were cultured with DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum, 100 IU/mL penicillin, and 100 μg/mL streptomycin, and maintained at 37°C in 5% CO2.
4
Cell Culture Protocols for Diverse Cell Types
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NCTC clone 929 (L929) cells (ATCC CCL-1) and adipose-derived mesenchymal stem cells (MSC)
(hMSC, ATCC PCS-500-011) were purchased from the ATCC. Primary rat hepatocytes were
obtained from Kurabo Industries, Japan.
L929 were cultured in MEM-NEAA medium supplemented with 10% horse serum, 2 mM
L-glutamine, and 1 mM sodium pyruvate. The hMSCs were cultured in MSC Basal Medium (ATCC
PSC-500-030) supplemented with MSC Growth Kit and 100 U/ml penicillin-0.1 mg/mL,
streptomycin-0.25 µg/ml, and amphotericin B (Biological industries). Rat primary
hepatocytes were cultured in Williams E medium (Sigma) supplemented with 10% inactivated
FBS (Biological industries), 2 mM L-glutamine (Sigma), 1.72 µM insulin (Lifetech), 0.07 µM
transferrin (Lifetech), 0.04 µM selenium (Lifetech), and 0.1 µM dexamethasone (Sigma).
(hMSC, ATCC PCS-500-011) were purchased from the ATCC. Primary rat hepatocytes were
obtained from Kurabo Industries, Japan.
L929 were cultured in MEM-NEAA medium supplemented with 10% horse serum, 2 mM
L-glutamine, and 1 mM sodium pyruvate. The hMSCs were cultured in MSC Basal Medium (ATCC
PSC-500-030) supplemented with MSC Growth Kit and 100 U/ml penicillin-0.1 mg/mL,
streptomycin-0.25 µg/ml, and amphotericin B (Biological industries). Rat primary
hepatocytes were cultured in Williams E medium (Sigma) supplemented with 10% inactivated
FBS (Biological industries), 2 mM L-glutamine (Sigma), 1.72 µM insulin (Lifetech), 0.07 µM
transferrin (Lifetech), 0.04 µM selenium (Lifetech), and 0.1 µM dexamethasone (Sigma).
5
Retrovirus-mediated gene expression in L929 cells
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Parental L929 cell line (ATCC® CCL-1™, purchased from ATCC) were infected with retrovirus containing JAK2WT, JAK2V617F, CALRDEL, CALRWT, MPL, or empty vector containing green fluorescent protein (GFP) or human CD4 (hCD4) tags by spinoculation. GFP+ and/or hCD4+ cells were sorted on an Aria flow cytometer (BD) and expanded in culture.
6
Zirconia Bioactivity and Cytocompatibility
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First, cytocompatibility of plain zirconia discs was evaluated in a pilot study (n=6), and no statistically significant differences in L929 cell viability were found when they were seeded on zirconia or polystyrene surfaces for up to 7 days (data not shown). Therefore, the following experimental groups were established for biological assays: Zr -zirconia discs; and Zr+BS -zirconia discs subjected to BS treatment (n=6). The samples were adapted to a metallic device between two silicon rings to keep the discs in position and to standardize the seeding area. The sets were sterilized in ethylene oxide and placed individually in wells of 24-well plates. Then, L929 mouse fibroblast cells Zirconia-coated bioactive glass (subcutaneous connective tissue; ATCC® CCL1™, Manassas, VI, USA) were seeded on material surfaces in Dulbecco's Modified Eagle's Medium (DMEM; supplemented with 100 IU/mL penicillin, 100 μg/mL streptomycin, and 2 mmol/L glutamine; GIBCO, Grand Island, NY, USA), supplemented with 10% fetal bovine serum (FBS; GIBCO). One 30-μL drop of culture medium containing 3 x 104 cells was applied exclusively to material surfaces, followed by a 30-min incubation time at 37 °C and 5% CO2, to allow for cell attachment in a 50% confluence pattern. Thereafter, 1 mL of culture medium was added to the sets, followed by further cultivation for up to 7 days.
7
Cytotoxicity Evaluation of EGCG in L929 Cells
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(−)-Epigallocatechin-3-gallate, 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), SPC, trypsin-EDTA solution, dimethyl sulfoxide (DMSO) for cell culture, and penicillin/streptomycin solution were purchased from Sigma, USA. Carbopol 980 was purchased from the Abdi İbrahim Pharmaceutical, Industry and Trade Company, Turkey. All the other chemicals used were of analytical grade. The NTCT clone 929 cell line (L929, connective mouse tissue) was acquired from the American Type Culture Collection (ATCC® CCL-1™), Manassas, USA. Eagle's Minimum Essential Medium (EMEM) (ATCC® 30-2003™) was purchased from Biochrom, Germany. Cell culture flasks and plates were purchased from Corning®. Cedex. Smart Slides and Trypan Blue solution were purchased from Roche (Switzerland). Mouse collagenase and elastase enzyme ELISA kits were obtained from Sunredbio, Shanghai.
8
Cultivation and Characterization of Cell Lines
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Cancer cell lines: BxPC-3 (pancreas adenocarcinoma, ATCC® CRL-1687TM), HCT-116 (colorectal carcinoma, ATCC® CCL-247™), PC-3 (prostate cancer, ATCC® CRL-1435TM), and normal cell lines: L929 (mouse fibroblast, ATCC® CCL-1™) and WI-38 (human lung fibroblasts, ATCC® CCL-75TM) were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA). Compositions of culture media used to cultivate cells are presented in Table 8 .
Cells were grown at 37 °C in a humidified atmosphere of 5% CO2 in the air. The culture medium was changed every 24–48 h. Subculture was performed using 0.25% trypsin/EDTA after cells reached confluence.
Cells were grown at 37 °C in a humidified atmosphere of 5% CO2 in the air. The culture medium was changed every 24–48 h. Subculture was performed using 0.25% trypsin/EDTA after cells reached confluence.
9
Cytocompatibility of h-BN_AuNP Nanocomposite
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Two adherent cell lines—murine L929 fibroblast (ATCC® CCL-1™, American Type Culture Collection, Manassas, VA, USA) and MCF-7 human breast adenocarcinoma (ATCC® HTB-22™, American Type Culture Collection, Manassas, VA, USA)—were chosen for cytocompatibility studies of the h-BN_AuNP nanocomposite.
For morphology analyses (phase contrast and holographic microscopy), cells of each line were seeded into T25 flasks (Sarstedt, Nümbrecht, Germany) and maintained in standard cell culture conditions at 37 °C, 5% CO2, 95% humidity. Complete Dulbecco’s Modified Eagle Medium (DMEM) culture medium supplemented with 10% heat inactivated fetal bovine serum (FBS) (PAA Laboratories GmbH, Pasching, Austria), 2 mMl -glutamine, 50 IU mL−1 penicillin and 50 µg mL−1 streptomycin (Sigma-Aldrich, St. Louis, MO, USA) was used in the study.
For cytocompatibility analysis, the cells were seeded into 96-well plates (Corning Inc., New York, NY, USA) and cultured for 24, 48 and 72 h in standard conditions mentioned above. All cell cultures were monitored with a Nikon TS-100 microscope (Nikon, Melville, NY, USA).
For morphology analyses (phase contrast and holographic microscopy), cells of each line were seeded into T25 flasks (Sarstedt, Nümbrecht, Germany) and maintained in standard cell culture conditions at 37 °C, 5% CO2, 95% humidity. Complete Dulbecco’s Modified Eagle Medium (DMEM) culture medium supplemented with 10% heat inactivated fetal bovine serum (FBS) (PAA Laboratories GmbH, Pasching, Austria), 2 mM
For cytocompatibility analysis, the cells were seeded into 96-well plates (Corning Inc., New York, NY, USA) and cultured for 24, 48 and 72 h in standard conditions mentioned above. All cell cultures were monitored with a Nikon TS-100 microscope (Nikon, Melville, NY, USA).
10
Virus Infection Assay in L929 Cells
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