The human GBM U87MG cell line, human brain endothelial hCMEC/D3 cell line, N2a cell line, BV2 cell line, U251 cell line, U251TR cell line were purchased from the the American Type Culture Collection (ATCC). U87MG-Luc cell line was purchased from Shanghai Model Organisms Center (Shanghai, China). The above cell lines were cultured in DMEM medium supplemented with 10% (V/V) fetal bovine serum and 1% (V/V) penicillin and streptomycin. The X01 glioblastoma stem cells (GSCs) were kindly provided by Professor Jong Bae Park from the National Cancer Center of South Korea, the more details are in the previous works49 (link),61 (link)–64 (link). X01 cells were maintained in DME/F12 supplemented with epidermal growth factor (EGF, 10 ng ml−1), basic fibroblast growth factor (bFGF, 10 ng ml−1), B27, and 1% penicillin and streptomycin. These cells were cultured as a monolayer in a humidified atmosphere containing 5% CO2 at 37 °C.
Bv2 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The BV2 cell line is a murine (mouse) microglial cell line derived from the olfactory bulb. Microglial cells are the resident macrophages of the central nervous system and play a crucial role in immune response and neuroinflammation. The BV2 cell line is commonly used as an in vitro model to study microglial cell function and behavior.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Lipopolysaccharides (lps), by Merck Group (2 mentions)
Dulbecco modified eagle medium (dmem), by Keygen Biotech (2 mentions)
U87mg luc cell line, by Shanghai Model Organisms (1 mentions)
N2a cell line, by American Type Culture Collection (1 mentions)
U87mg cell line, by American Type Culture Collection (1 mentions)
U251 cell line, by American Type Culture Collection (1 mentions)
Lps from escherichia coli 055 b5, by Merck Group (1 mentions)
Recombinant cxcl1, by R&D Systems (1 mentions)
Eclipse ti2, by Nikon (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Minimum essential media, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin and streptomycin, by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions)
10 protocols using bv2 cell line
1
Culture Conditions for Brain Cell Lines
2
Microglial Cell Migration Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Migration of BV-2 murine microglial cells was assessed with a transwell migration chamber. BV2 cell line was obtained from the American Type Culture Collection (Manassas, VA, USA). Migration was induced by recombinant CXCL1 (100 ng/ml, R&D systems, Catalog #: 275-GR-010/CF) or recombinant CX3CL1 (10 nM, Sino Biological, Catalog #: 10636-H08H) with or without LPS from Escherichia coli 055:B5 (100 ng/ml, Sigma-Aldrich) in the lower chamber. The number of cells in each well was normalized to the average number of cells in the control condition (100%)22 (link).
3
BV-2 Cell Culture and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BV-2 cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, United States). They were cultured in DMEM (KeyGen Biotech Co., Ltd., Nanjing, China). The medium was supplemented with 10% FBS (Gibco, Grand Island, NY, United States), 100 U/ml penicillin, and 100 mg/ml streptomycin, accompanied with 95% air/5% CO2 at 37°C. BV-2 cells were incubated with ACT (50, 25, and 12.5 μM) or stimulated with lipopolysaccharide (LPS, 1 μg/ml; Sigma-Aldrich, St Louis, MO, United States) for 24 h. The cells were observed under the inverted microscope (Nikon ECLIPSE Ti2, Japan).
4
Murine Microglial Cell Culture Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BV-2 cell line (immortalized murine microglial cell line) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). They were cultured in DMEM (KeyGen Biotech Co., Ltd, Nanjing, China). The medium was supplemented with 10% FBS (Gibco, Grand Island, NY, USA), 100 U/mL penicillin, as well as 100 mg/mL streptomycin, with 95% air/5% CO 2 at 37℃. BV-2 cells were incubated with ACT (50, 25, 12.5 μM) or stimulating with Lipopolysaccharide (LPS, 1 μg/mL; Sigma-Aldrich, St Louis, MO, USA) for 24 h. Finally, all the cells or supernatant were collected for the various analyses.
5
Eftud2 Knockdown in BV2 Microglia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BV2 cell line (RRID: CVCL_XD67) was obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in minimum essential media (Invitrogen, Waltham, MA, USA) containing 10% fetal bovine serum (Gibco, Grand Island, CA, USA) and 1% penicillin-streptomycin (Invitrogen). All cells were maintained at 37°C in a 5% CO2 environment. LPS (1 μg/μL; Sigma-Aldrich; dissolved in normal saline) was used to stimulate BV2 cells in vitro for 12 or 24 hours after transfection. BV2 cells were identified through immunostaining using anti-ionized calcium binding adapter molecule 1 (Iba1). The Eftud2 and negative control short interfering RNAs (siRNAs) were synthesized by GenePharma (Suzhou, China). Lipofectamine RNAimax (Invitrogen) was used to transfect the Eftud2 or negative control siRNAs into the BV2 cells according to the manufacturer’s instructions. Briefly, siRNA and transfection reagent were mixed at a ratio of 2:1 and were added to BV2 cells after standing for 20 minutes. To ensure stable knockdown of Eftud2 in BV2 cells, supernatants or cells were collected 3 days after siRNA transfection.
6
BV2 Cell Response to LPS and CAPE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BV2 cell lines were purchased from American Type Culture Collection. They were inoculated into culture flasks in appropriate concentrations, with DMEM containing 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin. Then, they were kept at 37°C in humidified 5% CO2 overnight, and the next morning, the medium was changed to serum-free medium and then the cells were treated with LPS (1 µg/mL) with or without CAPE (100 µM) for 12 hours. We used dimethyl sulfoxide (0.1%) as a control.
7
Rat Pheochromocytoma and Microglial Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rat adrenal pheochromocytoma cell lines (PC-12) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and BV2 cell lines was purchased from the American Type Culture Collection. Both cultured in Dulbecco's modified Eagle's medium (DMEM, high glucose, NanJing KeyGen Biotech Co., Ltd.) containing 10 % fetal bovine serum (FBS, Gibco), penicillin (100 IU/ml) and streptomycin (100 μg/ml). Cells were cultured in a humidified incubator with 5% CO2 at 37°C and medium was replaced every 2-3 days.
The PC-12 cells were adjusted to 2×105cell/well and were seeded in a 6-well plate. The cells were incubated with Sal (2, 10, 50 μM) for 2 h, followed by stimulating with MPTP (500 μM) for 24 h. Finally, all the cells were collected for the various analyses.
The BV2 cells were seeded in a 6-well plate at a density of 2×105cell/well. Then, cells were incubated with Sal (2, 10, 50 μM) for 2 h, followed by stimulating with LPS (100 ng/ml) for 30 min. Finally, all the cells were then collected for the various analyses.
The PC-12 cells were adjusted to 2×105cell/well and were seeded in a 6-well plate. The cells were incubated with Sal (2, 10, 50 μM) for 2 h, followed by stimulating with MPTP (500 μM) for 24 h. Finally, all the cells were collected for the various analyses.
The BV2 cells were seeded in a 6-well plate at a density of 2×105cell/well. Then, cells were incubated with Sal (2, 10, 50 μM) for 2 h, followed by stimulating with LPS (100 ng/ml) for 30 min. Finally, all the cells were then collected for the various analyses.
8
BV-2 Cell-based In Vitro Model of SCI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BV-2 cells were widely used to induce cell injuries to mimic the in vitro model of SCI [29 (link)]. The BV-2 cell line was obtained from ATCC (Manassas, VA, USA) and maintained in DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% FBS (Gibco), and 1% penicillin and streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in 5% CO2 at 37 °C.
For the induction of inflammation and apoptosis in the cultured BV-2 cells, 100 ng/ml of lipopolysaccharide (LPS) were used to stimulate cells (Sigma-Aldrich, St Lousis, MO, USA) for 4 h at 37 °C as previously reported [30 (link)].
For the induction of inflammation and apoptosis in the cultured BV-2 cells, 100 ng/ml of lipopolysaccharide (LPS) were used to stimulate cells (Sigma-Aldrich, St Lousis, MO, USA) for 4 h at 37 °C as previously reported [30 (link)].
9
Isolating and Culturing Brain Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells from the mice brain tissues were isolated as previously described (Zhou et al. 2016 (link)). BV2 cell line was purchased form ATCC, USA and recovered in our lab. Cells were divided into control group, high-glucose group, H/R group and high-glucose + H/R group. To establish the H/R cell model, the cells were cultured in glucose-free DMEM and incubated at 37 °C in a multi-gas incubator with 94% N2, 5% CO2 and 1% O2. Following hypoxia, Cells were incubated for another 4 h in standard DMEM at 37 °C with 5% CO2 for reoxygenation.
10
Cell Culture and Zebrafish Husbandry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The murine microglial BV-2 cell line and the photoreceptor-derived 661W cell line were purchased from ATCC and Guangzhou Jennio Biotech, respectively. Cells were cultured in Dulbecco's modified Eagle’s medium (DMEM; Biological Industries (BI), Israel) containing 10% (v/v) fetal bovine serum (FBS; BI) and 1% penicillin–streptomycin (Thermo Fisher Scientific). All cells were incubated at 37 °C with 5% CO2. Adult wild-type zebrafish (Tübingen strain, 3–6 months old) were obtained from the Institute of Hydrobiology, Chinese Academy of Sciences, and raised in a standard fish facility with a 14/10-h light/dark cycle at 28.5 °C [47 ]). Embryos or larvae were incubated in E3 medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, and 0.33 mM MgSO4, pH 7.2) and staged by hpf.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!