33 syringe pump

An isovolemic hemodilution was performed using PE consisting of a solution of Dextran 6% T2000 (Pharmacosmos, Holbaek, Denmark) in 0.9% sodium chloride mixed with 10% human serum albumin. This procedure has been reported elsewhere, briefly, forty percent (40%) of the animals blood volume (BV) estimated as 7% of body weight was withdrawn from the femoral artery catheter using a dual syringe pump (33 syringe pump, Harvard Apparatus, Holliston, MA, USA) at a rate of 100 µL/min.17) (link) Simultaneously, PE was administered into the jugular vein catheter at the same rate to lower the systemic Hct to about 20%. The experimental protocol is schematically shown in Fig. 1. The animals were monitored for 1 hour after the completion of the hemodilution. At the end of experiment, blood samples were collected to measure plasma viscosity, plasma colloid osmotic pressure (COP) and blood conductance. The PE solution had a viscosity of 6.3 cP and 43 mm Hg in COP.