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The Hs578T is a human breast cancer cell line derived from a carcinoma. It is a versatile cell line used in various cell-based assays and research applications.

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Lab products found in correlation

Mda mb 231, by National Collection of Authenticated Cell Cultures (11 mentions) Mcf 7, by National Collection of Authenticated Cell Cultures (9 mentions) Sk br 3, by National Collection of Authenticated Cell Cultures (7 mentions) Bt 549, by National Collection of Authenticated Cell Cultures (6 mentions) Mcf 10a, by National Collection of Authenticated Cell Cultures (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Hcc1937, by National Collection of Authenticated Cell Cultures (3 mentions) Bt 474, by National Collection of Authenticated Cell Cultures (3 mentions) Mcf 10a, by Lonza (2 mentions) Gv493 lentiviral shrna vector, by Genechem (2 mentions) Mda mb 468, by National Collection of Authenticated Cell Cultures (2 mentions) Mda mb 231, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dmem medium, by Procell (1 mentions) Hs578t, by Procell (1 mentions) Thp 1, by National Collection of Authenticated Cell Cultures (1 mentions) Human umbilical vein endothelial cells (huvec), by National Collection of Authenticated Cell Cultures (1 mentions) Rpmi 1640 medium, by Procell (1 mentions) Mda mb 231, by Procell (1 mentions)

13 protocols using hs578t

1

Culturing Diverse Human Cell Lines

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The human breast cell line MCF-10 A, human breast cancer cell lines (MCF7, T47D, SKBR-3, MDA-MB-231 and Hs578T), human monocyte cell line THP-1 and human umbilical vein endothelial cell line HUVEC were all purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). T47D, SKBR3, MCF7, MDA-MB-231, Hs578T and HUVEC cells were cultured with DMEM medium (Procell, China) containing 10% fetal bovine serum (FBS). THP-1 cells were cultured with RPMI-1640 medium (Procell, China) with 10% FBS and 0.05 mM ß-Mercaptoethanol. MCF-10 A cells cultured with MEGM kit (Lonza Clonetics, Switzerland). All cells were cultured at 37 °C and in 5% CO2 humidified atmosphere incubator.
Xu D., Liu Z., Liang M.X., Chen W.Q., Fei Y., Yang S.J., Wu Y., Zhang W, & Tang J.H. (2023). Hyperthermia promotes M1 polarization of macrophages via exosome-mediated HSPB8 transfer in triple negative breast cancer. Discover. Oncology, 14, 81.
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2

Culturing Authenticated Mammary Cell Lines

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Human non-transformed mammary epithelial cell line MCF-10A and breast cancer cell lines, including MCF-7, ZR-75-1, SK-BR-3, MDA-MB-231, BT549, Hs578t, in this study were purchased from National Collection of Authenticated Cell Cultures (China). All cell lines were cultured under 37°C, 5% CO2 condition.
Yuan R., Zhang Y., Wang Y., Chen H., Zhang R., Hu Z., Chai C, & Chen T. (2023). GNPNAT1 is a potential biomarker correlated with immune infiltration and immunotherapy outcome in breast cancer. Frontiers in Immunology, 14, 1152678.
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3

TNBC Cell Line Manipulation and Lentiviral Transduction

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The human TNBC cell lines MDA-MB-231, BT-549, HCC1937, and Hs578T were purchased from the Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. Cell were cultured in Dulbecco's Modified Eagle Medium (DMEM; HyClone, Logan, USA), supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA) and 1% penicillin and streptomycin solution and incubated at 37 °C and 5% CO2. The short hairpin RNA (shRNA) targeting YTHDF3 was subcloned into the GV493 lentiviral shRNA vector (Genechem, Shanghai, China). For overexpression of ZEB1, the construct was generated by subcloning PCR amplified full-length human ZEB1 cDNA into the vector. The constructed lentiviral vectors were packaged into viruses in 293 T cells which were then harvested and the concentrated viruses were added to TNBC cells and cultured for 72 hours. Gene sequences are provided in Table S1.
Lin Y., Jin X., Nie Q., Chen M., Guo W., Chen L., Li Y., Chen X., Zhang W., Chen H., Jiang M., Xiao H., Zhang J., Fu F, & Wang C. (2022). YTHDF3 facilitates triple-negative breast cancer progression and metastasis by stabilizing ZEB1 mRNA in an m6A-dependent manner. Annals of Translational Medicine, 10(2), 83.
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4

Lentiviral Transduction of Breast Cancer Cells

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Human breast cancer cell lines (MDA-MB-231, HCC1937, BT-549, Hs578T, SKBR-3 and MCF-7) were purchased from the Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. All cell lines were cultured in DMEM (HyClone) supplemented with 10% FBS (Gibco) and 1% penicillin and streptomycin solution at 37 °C under 5% CO2 conditions in a humidified incubator. Short hairpin RNA (shRNA) targeting CTPS1 were subcloned into GV115 and GV493 lentiviral shRNA vector (Genechem, Shanghai, China), respectively. For overexpressing YBX1, the construct was generated by subcloning PCR amplified full-length human YBX1 cDNA into the GV657 vector (Genechem, Shanghai, China). The constructed lentiviral vectors were packaged into the viruses in 293 T cells. Then, the harvested and concentrated viruses were added into cells and cultured for 72 h. The target sequences of the shRNA and negative control were as follows:
shCTPS1-1: 5’-ATCTTGTAGCGGATGATTC-3’.
shCTPS1-2: 5’-GAGGATTTGGTGTTCGAGGA-3’.
shCtrl: 5’-TTCTCCGAACGTGTCACGT-3’.
Lin Y., Zhang J., Li Y., Guo W., Chen L., Chen M., Chen X., Zhang W., Jin X., Jiang M., Xiao H., Wang C., Song C, & Fu F. (2022). CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1. Journal of Translational Medicine, 20, 17.
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5

Manipulation of β-Catenin in TNBC Cells

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TNBC MDA-MB-231, MDA-MB-468 and Hs578T cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in high glucose DMEM medium containing 10% fetal bovine serum (FBS), 2 mM of L-glutamine, 100 units/ml of penicillin, and 100 μg/ml of streptomycin under standard cell culture conditions at 37°C in a humidified atmosphere with 5% CO2. TNBC cells were transfected with β-catenin shRNA, β-catenin cDNA and the corresponding empty vector plasmids using Lipofectamine® 2000 (Invitrogen/ThermoFisher Scientific, Grand Island, NY) according to manufacturer's specifications, respectively. β-catenin shRNA cloned in pLKO.1 vector, β-catenin cloned in pcDNA3 vector and the corresponding empty vector plasmids were obtained from Addgene (Cambridge, MA).
Yin L., Gao Y., Zhang X., Wang J., Ding D., Zhang Y., Zhang J, & Chen H. (2016). Niclosamide sensitizes triple-negative breast cancer cells to ionizing radiation in association with the inhibition of Wnt/β-catenin signaling. Oncotarget, 7(27), 42126-42138.
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6

Breast Cancer Cell Line Cultivation

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The cell lines BT549, HS578T, MCF‐7, and MDA‐MB‐231 were obtained from Cell Bank of the Chinese Academy of Sciences, which were cultivated in a 37°C incubator under the conditions of 5% CO2 and 95% humid air. Breast cancer cell lines BT549 and MDA‐MB‐231 were cultured in RPMI medium supplemented with 10% FBS and 100 μg/ml penicillin‐streptomycin. HS578T and MCF‐7 cells were maintained in MEM containing 10% FBS, 100 μg/ml streptomycin, and 100 U/ml of penicillin G.
He J., Gao R., Yang J., Li F., Fu Y., Cui J., Liu X., Huang K., Guo Q., Zhou Z, & Wei W. (2022). NCAPD2 promotes breast cancer progression through E2F1 transcriptional regulation of CDK1. Cancer Science, 114(3), 896-907.
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7

Ethical Breast Cancer Sample Extraction

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This study was conducted in accordance with the principles stated in the Declaration of Helsinki (as revised in 2013). Ethical clearance was granted from the First Affiliated Hospital of Nanjing Medical University Medical Science Research Ethics Committee (Ethics code: 2021-SR-308). Breast cancer specimens were harvested from the Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, and written informed consent was provided by the patients or their next of kin. Surgical specimens were resected, snap-frozen in liquid nitrogen, and deposited in the Biological Sample Bank of Jiangsu Province Hospital. The catalogue number of samples used in this study were 399, 459, 461, 474, 476, 477 and 488. The breast tumor cells MCF-7, ZR-75-1, BT-474, SK-BR-3, MDA-MB-453, MDA-MB-231, BT-549, Hs-578T, HCC1806, and HCC1937 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). For western blot analysis, frozen human breast cancer samples were ground and homogenized in a mortar and pestle under liquid nitrogen and then lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Solarbio, Beijing, China) supplemented with protease inhibitors phenylmethylsulfonyl fluoride (PMSF; Beyotime, Shanghai, China). Cells were rinsed twice with phosphate-buffered saline (PBS) and lysed in RIPA buffer on ice.
Fang Z., Song Y.X., Wo G.Q., Zhou H.L., Li L., Yang S.Y., Chen X., Zhang J, & Tang J.H. (2022). Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer. Annals of Translational Medicine, 10(23), 1280.
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8

Culturing Human Breast Cancer Cell Lines

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The human breast cancer BT474, SKBR3, Hs578T and MDA-MB-231 cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The Hs578T cell line was cultured in highglucose DMEM (Gibco, Grand Island, NY, USA) supplemented with 10% foetal bovine serum (FBS) (Gibco) and 0.01 mg/ml insulin (Invitrogen, Carlsbad, CA, USA). BT474 cells were grown in high-glucose DMEM supplemented with 10% FBS. SKBR3 and MDA-MB-231 cell lines were cultivated in RPMI-1640 medium (Gibco) supplemented with 10% FBS. All cells were incubated in a humidified incubator at 37˚C with a 5% CO 2 atmosphere and maintained in a logarithmic growth phase for all the experiments.
Zou M., Li Y., Xia S., Chu Q., Xiao X., Qiu H., Chen Y., Zheng Z., Liu F., Zhuang L, & Yu S. (2017). Knockdown of CAVEOLIN-1 Sensitizes Human Basal-Like Triple-Negative Breast Cancer Cells to Radiation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(2).
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9

Nischarin's Effects on Triple-Negative Breast Cancer

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Hormone receptor positive (MCF-7), HER2 positive (SKBR3) and two triple negative breast cancer (MDA-MB-231 and Hs578T) cell lines, were purchased from the Cell Bank of the Chinese Academy of Sciences. The cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal bovine serum (FBS) and 100 U/ml penicillin/streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37°C and 5% CO2. As the primary goal of the present study was to study the effects of Nischarin on triple-negative breast cancer cells, only the two triple negative breast cancer (MDA-MB-231 and Hs578T) cell lines were used in the next experiment.
Cai Y.J., Ma B., Wang M.L., Chen J., Zhao F.G., Zhou J.D., Guo X., Zheng L., Xu C.J., Wang Y., He Y.B., Liu J, & Xie S.N. (2020). Impact of Nischarin on EMT regulators in breast cancer cell lines. Oncology Letters, 20(6), 291.
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10

Breast Cancer Cell Line Characterization

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MCF 10A cells and the human breast cancer cell line T-47D were purchased from ATCC (Gaithersburg, Maryland), and other cell lines, including MCF-7, BT-474, SK-BR-3, MDA-MB-231, MDA-MB-468, BT-549, and Hs 578T, were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cell lines used were analyzed by short tandem repeat profiling, and the culture conditions of these cell lines followed ATCC protocols.
Wu S., Guo X., Zhou J., Zhu X., Chen H., Zhang K., Lu Y, & Chen Y. (2020). High expression of UNC5B enhances tumor proliferation, increases metastasis, and worsens prognosis in breast cancer. Aging (Albany NY), 12(17), 17079-17098.
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