Porous Vycor glass (Corning 7930) was purchased from Advanced Glass and Ceramics (Holden, MA). Silicon phthalocyanine dichloride, aluminum(III) phthalocyanine chloride tetrasulfonic acid, 9-bromo-2-methylnon-2-ene, sodium sulfite, triphenyl phosphine (PPh3), benzoic acid, dimethylsulfone, DMF, CH2Cl2, ethanol, D2O, and DMSO-d6 were purchased from commercial suppliers and were used as received. Dichloromethane was distilled over phosphorus pentoxide prior to use. Deionized water was purified with a U.S. Filter Corporation deionization system (Vineland, NJ). Nuclear magnetic resonance (NMR) data were recorded on a Bruker Avance 400 spectrometer operating at 400 MHz for 1H NMR and at 100.6 MHz for 13C NMR. UV–vis data were collected on a Hitachi UV–vis U-2001 instrument. FAB-mass spectrometry data were collected on a JEOL JMS-HX110 spectrometer using a m-nitrobenzyl alcohol matrix, a 10 kV acceleration voltage, and a Xe beam FAB gun (6 kV) on the MS-1 ion source. Infrared spectra were recorded on a Nicolet iS10 FT-IR spectrometer. Solution temperatures were measured with a digital pyrometer (Thermo Scientific). An Olympus FluoView FV10i confocal fluorescence microscope was used to analyze stained E. coli and assess membrane permeability following singlet oxygen exposure.
Jms hx110 spectrometer
Manufactured by JEOL
Sourced in United States
The JMS-HX110 is a double-focusing magnetic sector mass spectrometer designed for high-resolution analysis. It features a Nier-type ion source and a Mattauch-Herzog geometry analyzer. The instrument is capable of providing accurate mass measurements and detailed structural information for a wide range of organic and inorganic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Silica gel 60 pf254 cards, by Merck Group (2 mentions)
Fluoview fv10i confocal fluorescence microscope, by Olympus (1 mentions)
Avance 400 spectrometer, by Bruker (1 mentions)
Melting point b 540 apparatus, by Büchi (1 mentions)
Cdcl3, by Bruker (1 mentions)
B 540, by Büchi (1 mentions)
Sio2 60, by Merck Group (1 mentions)
4 protocols using jms hx110 spectrometer
1
Porous Glass Photosensitizer Synthesis
2
Characterization of Organic Compounds
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Melting points were determined with help of (Buchi B-540) melting point apparatus (Buchi, New Castle, DE, USA). Proton (1H) NMR and Carbon (13C) NMR spectra were obtained in CDCl3 at 500/126 MHz (Bruker, Billercia, MA, USA), respectively. EI-MS spectra were obtained on JMS-HX-110 spectrometer (JEOL, Peabody, MA, USA). Silica gel (70–230 mesh) was used for purification of compounds in column chromatography. The reactions were monitored on TLC, using Merck silica gel 60 PF254 cards. Visualization of compounds was done by using UV lamp (254–365 nm).
3
Characterization of Synthesized Products
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The Buchi B-540 was used for determining the melting points of the synthesized products (Buchi, New Castle, DE, USA). 1H-NMR and 13C-NMR spectra were obtained in chloroform-d at 500/126, 400/100, MHz (Bruker, Billerica, MA, USA). The EI-MS spectra were recorded using the JMS-HX-110 spectrometer (JEOL, Peabody, MA, USA). TLC (Merck silica gel 60 PF254 cards) was used for monitoring the reaction. A UV lamp was used for visualization of the compounds (254–365 nm). The dry ethanol used in the experiments was dried by following a previously reported method [24 (link)].
4
General Analytical Procedures for Organic Synthesis
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The reactions were performed under an air atmosphere unless otherwise stated. All solvents and reagents were employed as received. Analytical thin layer chromatography (TLC) was performed on SiO2 60 F254 plates and flash column chromatography was carried out using SiO2 60 (particle size 0.040–0.055 mm, 230–400 mesh), both of which are available from E. Merck (Darmstadt, Germany). Visualization was performed under UV irradiation at 254 nm followed by staining with aqueous potassium permanganate [KMnO4 (3 g) and K2CO3 (20 g) in 300 mL of H2O containing 5 mL of an aqueous solution of NaOH (5%, w/v)] and charring by heat gun. 1H- and 13C-NMR spectra were recorded on a 500 FT NMR instrument (Bruker, Billerica, MA, USA). Chloroform-d and methanol-d were used as solvents and TMS (δ = 0.00 ppm) as an internal standard. Chemical shifts are reported as δ values in ppm as referenced to TMS. Multiplicities are recorded as s (singlet), d (doublet), t (triplet), q (quartet), quint (quintet), sext (sextet), sept (septet), dd (doublet of doublets), dt (doublet of triplets), br (broad), m (multiplet). Coupling constants (J) are expressed in Hz. LRMS and HRMS were measured by a JMS-HX110 spectrometer (JEOL, Tokyo, Japan) and spectroscopic data were recorded as m/z values. Melting points were measured using an Electrothermal instrument (Anatec Yanaco Inc., Kyoto, Japan).
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