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Anti human cd24 pe clone ebiosn3

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Anti-Human CD24 PE Clone: eBioSN3 is a flow cytometry reagent. It is a phycoerythrin (PE)-conjugated monoclonal antibody that binds to the human CD24 cell surface antigen.

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2 protocols using anti human cd24 pe clone ebiosn3

1

CD44+hi/CD24-low CSC Identification

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Samples were prepared with staining for CD44 using Mouse Anti-Human CD44 (Pgp-1) Cat# GWB-1F90D6 (GenWay Biotech, San Diego, CA, USA) and for CD24 using Anti-Human CD24 PE Clone: eBioSN3 Cat#12-0247-42 (eBioscience, San Diego, CA, USA) according to manufacture protocol. All samples were control stained for live cells with FxCycle Violet Stain Cat# F10347 (Life Technologies, Grand Island, NY, USA). Flow cytometry was performed on a FACS (fluorescence-activated cell sorting) DiVa (Becton Dickinson, Franklin Lakes, NJ, USA) machine. Cells were first gated around the Fx Violet, followed by side and forward scatter profiles to eliminate adherent cells. Gating thresholds were established for CD44 and CD24 positivity in order to analyze for the CD44+hi/CD24−low putative cancer stem cell populations.
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2

CD44+hi/CD24-low CSC Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were prepared with staining for CD44 using Mouse Anti-Human CD44 (Pgp-1) Cat# GWB-1F90D6 (GenWay Biotech, San Diego, CA, USA) and for CD24 using Anti-Human CD24 PE Clone: eBioSN3 Cat#12-0247-42 (eBioscience, San Diego, CA, USA) according to manufacture protocol. All samples were control stained for live cells with FxCycle Violet Stain Cat# F10347 (Life Technologies, Grand Island, NY, USA). Flow cytometry was performed on a FACS (fluorescence-activated cell sorting) DiVa (Becton Dickinson, Franklin Lakes, NJ, USA) machine. Cells were first gated around the Fx Violet, followed by side and forward scatter profiles to eliminate adherent cells. Gating thresholds were established for CD44 and CD24 positivity in order to analyze for the CD44+hi/CD24−low putative cancer stem cell populations.
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