A glucose assay kit (Abnova, Taiwan, China) and a lactate assay kit (KeyGen, Nanjing, China) were employed to test the levels of glucose consumption and lactate production in U251 and A172 cells. The cells (1 × 105/well) were plated in 12-well plates and incubated overnight. The glucose consumption and lactate production were monitored by the corresponding kits according to the manufacturer’s instructions.
Glucose assay kit
Manufactured by Abnova
Sourced in China
The Glucose assay kit is a laboratory equipment designed to measure the concentration of glucose in a sample. It is a colorimetric assay that uses a specific enzymatic reaction to quantify the amount of glucose present.
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Lab products found in correlation
3 protocols using glucose assay kit
1
Glucose and Lactate Assay in U251 and A172 Cells
2
Biochemical Analysis of Metabolic Markers
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Alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and
total cholesterol (TC) concentrations were determined using biochemical kits and methods
as described by Zhang et al.16 (link). Triglycerides were assayed using a triglyceride assay kit
(Triglyceride GPO-POD assay kit, Shanghai Zhen Biotechnology Co., Shanghai, China), and
cholesterol was assayed with a rat cholesterol assay kit (Catalog # 79960, Crystal Chem
USA, Elk Grove Village, IL, USA). A rat Alanine Aminotransferase (ALT/GPT) ELISA Kit was
purchased from Qayee Bio-Technology Co., Ltd. (Catalog #QY-D0179, Shanghai, China). A rat
Aspartate Aminotransferase (AST) ELISA kit was purchased from Cusabio Technology LLC (Code
CSB-E13023r, Wuhan, China). Leptin was detected with a Rat ELISA Kit (Catalog #KRC2281,
Thermo life, Shanghai, China). Glucose was detected with a Glucose Assay kit (Catalog
#KA4088, Abnova, Wuhan, China).
total cholesterol (TC) concentrations were determined using biochemical kits and methods
as described by Zhang et al.16 (link). Triglycerides were assayed using a triglyceride assay kit
(Triglyceride GPO-POD assay kit, Shanghai Zhen Biotechnology Co., Shanghai, China), and
cholesterol was assayed with a rat cholesterol assay kit (Catalog # 79960, Crystal Chem
USA, Elk Grove Village, IL, USA). A rat Alanine Aminotransferase (ALT/GPT) ELISA Kit was
purchased from Qayee Bio-Technology Co., Ltd. (Catalog #QY-D0179, Shanghai, China). A rat
Aspartate Aminotransferase (AST) ELISA kit was purchased from Cusabio Technology LLC (Code
CSB-E13023r, Wuhan, China). Leptin was detected with a Rat ELISA Kit (Catalog #KRC2281,
Thermo life, Shanghai, China). Glucose was detected with a Glucose Assay kit (Catalog
#KA4088, Abnova, Wuhan, China).
3
Glucose and Lactate Quantification
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Glucose and lactate concentrations were measured from supernatants using a colorimetric assay. The Glucose Assay Kit and the Lactate Assay Kit were purchased from Abnova (KA0831 and KA0833) and used according to the manufacturer’s recommendations. To fit into the linear concentration range of the assay, samples were diluted at 1:200 for glucose assay and 1:50 for lactate assay. Data were acquired using a Fluostar OPTIMA BMG Labtech plate reader and analyzed with the optima data analysis software (version 3.32).
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